Animal Core (Core D)

总结：动物核心（核心D） 对脑肿瘤生物学和治疗的研究最好在原位动物模型中进行， 肿瘤的自然环境从这些体内研究中得出有意义的结论的能力大大提高了 当使用均匀的、容易再现的动物模型时，核心还提供支持 研究人员使用基因工程小鼠模型（特别是RCAS/Ntv-a模型）。的目的 动物中心提供集中专业知识的动物模型服务，从而实现统一 和可重复性，允许实验，研究小组和项目之间的准确比较， 这个脑癌孢子在当前的资助期内，动物核心进行了546项实验， 超过15，000只动物的孢子研究人员。在接下来的5年资助期内，动物芯将继续 为脑癌孢子的所有研究者提供专家服务。为此，核心将： 1)为使用患者源性胶质瘤干细胞系（GSC）的原位动物实验提供支持， 其目前被认为是人神经胶质瘤体内模型的金标准。这个核心有 开发了大约100种GSC，其中40多种已经完全分子表征; 2)为使用患者来源的肿瘤外植体的侧腹和原位动物实验提供支持， 手术室植入NOD. Cg-PrkdcscidIl 2 rgtm 1 Wjl/SzJ（NSG）小鼠; 3)为使用免疫活性基因工程小鼠模型的动物实验提供支持 （GEMM），特别是RCAS/Ntv-a系统; 4)为原位动物实验提供支持，使用免疫活性 允许病毒复制的仓鼠模型; 5)为使用传统专业胶质瘤细胞系的原位动物实验提供支持， 人（U87、U251、LN 229）和小鼠（GL 261）; 6)为生物发光成像（BLI）或磁共振的体内成像提供支持 MRI检查以评估治疗策略的功效。 这些服务是使命关键型服务，并且本SPORE中的所有项目均使用其中一项或多项服务。

SUMMARY: ANIMAL CORE (CORE D) Investigations into brain tumor biology and therapy are best carried out in orthotopic animal models that mimic the natural milieu of the tumor. The ability to reach meaningful conclusions from these in vivo studies is greatly enhanced when uniform, readily reproducible animal models are used. The core also provides support for investigators using genetically engineered mouse models (in particular the RCAS/Ntv-a model). The purpose of the Animal Core is to provide an animal modeling service that centralizes expertise thereby achieving uniformity and reproducibility that permits accurate comparisons between experiments, research groups, and projects in this Brain Cancer SPORE. In the current funding period, the Animal Core performed 546 experiments involving over 15,000 animals for SPORE investigators. In the next 5-year funding period, the Animal Core will continue to provide expert services to all investigators in Brain Cancer SPORE. To this end, the Core will: 1) Provide support for orthotopic animal experiments using patient-derived glioma stem cell lines (GSCs), which are currently considered the gold standard in vivo model of human gliomas. This Core has developed approximately 100 GSCs, over 40 of which have been fully molecularly characterized; 2) Provide support for flank and orthotopic animal experiments using patient-derived tumor explants from the operating room implanted into NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice; 3) Provide support for animal experiments using immunocompetent genetically engineered mouse models (GEMMs), particularly the RCAS/Ntv-a system; 4) Provide support for orthotopic animal experiments testing oncolytic adenoviruses using immunocompetent hamster models that are permissive to viral replication; 5) Provide support for orthotopic animal experiments using traditional profession glioma cell lines from humans (U87, U251, LN229) and mice (GL261); 6) Provide support for in vivo imaging either with bioluminescence imaging (BLI) or magnetic resonance imaging (MRI) to assess efficacy of therapeutic strategies. These services are mission critical and one or more of these services are used by all Projects in this SPORE.