Core 4: Animal Breeding (UAB) and Exposure Core (MRIGLOBAL)

UAB研究中心的动物和暴露核心-4的主要目标 在军火库方面的卓越表现是支持所有科学项目和药物发现以及 开发核心-3，因为在本中心申请范围内提出的研究取得成功 取决于是否能及时获得足够的动物，以及 与核磁共振全球公司就动物暴露在军火库中进行协调。MRIGlobal有60多个 具有多年的化学试剂设施管理和运营经验 关联项目。我们为CACT资助的项目带来了对小动物和大动物的熟练程度 化学战剂(CWA)的模型、试验和评价及安全操作 化合物、剧毒化合物、有毒工业化学品(TIC)和新兴 化学威胁。CORE-4将通过评估 每年年初对每个项目的要求。动物将被运送到 在预定义的时间表上执行MRIGlobal。一旦这些动物暴露在军火库中 以及他们与皮肤损伤有关的初始总数据，这些动物将被实施安乐死 MRIGlobal的工作人员以及收获的组织和体液将被运回UAB。 此外，核磁共振全球公司将合成和储存用于这些动物研究的武器库，以便 他们拥有经过适当机构批准的设施。CORE-4将建立一个 UAB园区的生物库，在其中存储返回的组织和体液样本 以及项目直接生成的其他样本。样品将被条形码和银行储存 在我们的实验室里。此外，Core-4将对符合以下条件的药物进行体内筛选 由药物发现和开发核心-3合成，发现对 在体外减弱分子靶标。因此，这一核心的三个具体目标包括：1 根据批准的动物规程在MRIGlobal繁殖和运输用于军火库暴露的动物 UAB和MRIGlobal；2：与MRIGlobal协调动物接触军火药的问题，最初 大体皮肤数据收集，并将样品或治愈的动物退还给UAB，以获得适当的 分别进行储存和分配或进一步毒性评估；以及3：在体内进行 药物发现和开发核心-3中开发的新药的筛选。

The major objectives of the Animal and Exposure Core-4 of the UAB Research Center of Excellence in Arsenicals are to support all scientific Projects and Drug Discovery and Development Core-3, as the success of the studies proposed within this Center application depend on the availability of sufficient animals in a timely manner, as well as the timely coordination with MRIGlobal for animal exposure to arsenicals. MRIGlobal has more than 60 years of corporate experience in management and operations of chemical agent facilities and associated projects. We bring to CACT-funded programs, proficiencies in small and large animal chemical agent models, test and evaluation, safe handling of chemical warfare agent (CWA) compounds, compounds with extreme toxicity, toxic industrial chemicals (TIC), and emerging chemical threats. Core-4 will generate a colony of animals of the desired size by assessing the requirement of each Project in the beginning of each year. Animals will be transported to MRIGlobal on a predefined time schedule. Once the animals have been exposed to arsenicals and their initial gross data pertaining to skin damage recorded, the animals will be euthanized by the staff at MRIGlobal and the harvested tissues and body fluids will be transported back to UAB. In addition, MRIGlobal will synthesize and store the arsenicals used for these animal studies for which they have facilities which approved by appropriate agencies. Core-4 will establish a biorepository on the UAB campus in which to store the returned tissue and body fluid samples as well as other samples generated directly by the Projects. Samples will be barcoded and banked in our laboratory. In addition, Core-4 will conduct the in vivo screening of agents that are synthesized by Drug Discovery and Development Core-3 and found to be highly effective in attenuating molecular targets in vitro. Accordingly, three specific aims of this core include: 1: To breed and ship animals for arsenicals exposure at MRIGlobal under approved animal protocols of UAB and MRIGlobal; 2: Coordinate with MRIGlobal for animal exposure to arsenicals, initial gross skin data collection, and return shipment to UAB of samples or healed animals for proper banking and distribution or further toxicity assessment, respectively; and 3: To perform in vivo screening of novel agents developed in Drug Discovery and Development Core-3.