Multifunctional Nanoplatforms-Based Capture and identification of Circulating Tumor Biomarkers for Early Diagnosis of Lung Cancer

据报道，肺癌的发病率和死亡率有很大的差异。 在种族和种族多元化的人口中。非裔美国人患有肺病 癌症的发病率比其他任何人群都高。肺癌的高死亡率是由于 在治疗选择有限的较晚阶段被诊断出来。我们的长期合作 目标是为开发基于纳米平台的新型器件奠定基础 将提供更准确的循环癌症生物标记物的测定 肺癌患者在血液筛查设施中。这项建议的目的是 设计基于磁性荧光多功能纳米平台的捕获装置， 分离和检测循环肿瘤细胞(CTCs)和肿瘤来源的外切体 肺癌患者的血液样本，并建立这些发现之间的相关性 与生存有关。我们的中心假设是生物共轭多色荧光 附着碳点的磁性纳米平台可用于捕获多种类型 从血液样本中提取CTCs和循环外切体，并作为一种简单的多种颜色 用于准确识别循环生物标志物的荧光探头。中环 假设将通过追求两个具体目标来检验：1)发展荧光-- 磁性纳米平台，用于捕获和鉴定多种类型的CTCs 肺癌患者血液和2)肿瘤来源的捕获和鉴定 利用纳米平台的外切体及其临床应用示范。这个 提出的研究具有重要意义，因为它具有很高的灵敏度和实时性 检测的可能性，硫化镉附着的磁性纳米平台将非常有前途 用于检测和鉴定CTC的几种亚群类型和 外显体。这项工作的最接近的预期结果是提高能力 在血液筛查中识别多个临床相关的血液生物标志物 设施。

Significant disparities in incidence and mortality rates for lung cancer are reported among ethnically and racially diverse population. African Americans suffer from lung cancer more than any other population. High mortality rate in lung cancer is due to being diagnosed at a later stage, where treatment options are limited. Our long-term goal is to lay the groundwork for developing novel nanoplatform based device which will provide a more accurate determination of the circulating cancer biomarkers from lung cancer patients in blood screening facilities. The objective of this proposal is to design magnetic-fluorescence multifunctional nanoplatform based device to capture, isolate and detect circulating tumor cells (CTCs) and tumor derived exosomes from blood samples in lung cancer patients and to establish correlations of these findings with survival. Our central hypothesis is that bio-conjugated multicolor fluorescence carbon dots attached magnetic nano-platform can be used to capture multiple types of CTCs and circulating exosomes from blood samples, and as a facile multicolor fluorescence probe for accurate identification of circulating biomarkers. The central hypothesis will be tested by pursuing two specific aims: 1) Developing fluorescence- magnetic nanoplatform for capture, and identification of multiple types of CTCs from lung cancer patient blood and 2) Capture and identification of tumor derived exosomes using nanoplatform and demonstration of clinical applications. The proposed research is significant, because with high sensitivity and real-time detection possibility, CDs attached magnetic nano-platform will be highly promising for detection and identification of several subpopulation types of CTCs and exosomes. The proximate expected outcome of this work is improving the capability of identifying multiple clinically relevant blood based biomarkers in blood screening facilities.