CORE C- Protein Expression and Purification

项目总结/摘要 拟议的动物模型和组织分析核心（核心C）是一个重要组成部分 实现这个PPG的目标。支持个人的研究策略和成功 在本PPG申请中提出的项目中，核心C将为PPG的3个项目中的每个项目提供服务 用各种动脉血栓形成、动脉粥样硬化、静脉血栓形成和 自发性和降植烷诱导的系统性红斑狼疮（SLE）。此外还将 为3个项目中的每个项目提供全面的组织、血液和尿液样本分析。 此外，核心C将培训各个项目的研究员和技术人员， 动物研究。最后，核心C可以访问大学的档案FFPE标本 由核心C的联合研究者Gilmore博士监督的医院，他将确保所有 符合IRB对废弃组织研究的要求。吉尔摩医生会找到 用于PPG研究的适当组织，并为核心C提供必要的临床 信息，以便于分析。核心C将提供的服务需要专门的 技术技能和设备。核心C将减轻每个研究者的需要， 在各自的实验室建立技术，从而提高效率， 实验变化，并提供规模经济。的主要优势和创新 核心C是提供一个专门的，高度熟练的，可访问的和成本效益的资源， 动物模型和组织分析。 拟定核心C在心血管疾病动物模型方面具有丰富的经验 研究重点是各种疾病模型，包括血栓形成，动脉粥样硬化，血管炎， 中风、心脏肥大、小鼠组织和体液样本的分析和定量。 模型和分析已经建立并发表（参见核心C的生物技术 leader）。基于动物模型和组织分析的经验，Core C还 建立了自发的和降植烷诱导的SLE模型（参见图6和7）。 研究计划）。核心C将由王云梅博士领导，他在以下方面拥有丰富的经验： 血管损伤、血栓形成、动脉粥样硬化、中风和心脏肥大的鼠模型。 此外，王博士超过25年的动物研究经验， 如果核心C需要的话，她很好地为任何额外的专业知识和技能，涉及动物。芯C 一旦有了创新的动物模型， 适合项目的目的。此外，王博士还与所有 PPG的项目负责人（见Biosketch出版物）。

Project Summary/Abstract The proposed Animal Models and Tissue Analysis Core (Core C) is an essential component to the goals of this PPG. To support the research strategies and success of the individual projects proposed in this PPG application, Core C will serve each of the 3 projects of the PPG with a variety of animal models of arterial thrombosis, atherosclerosis, venous thrombosis, and spontaneous and pristane-induced systematic lupus erythematosus (SLE). In addition, it will provide comprehensive tissue, blood and urine sample analysis for each of the 3 projects. Furthermore, Core C will train fellows and technical staff of the individual projects to perform animal studies. Lastly, Core C has access to archival FFPE specimens at the University Hospitals that is overseen by Dr. Gilmore, a Co-Investigator of Core C, who will ensure that all IRB requirements for discarded tissue research have been met. Dr. Gilmore will locate appropriate tissues for the research of the PPG and provide Core C with the necessary clinical information to facilitate the analyses. The services Core C will provide require specialized technical skills and equipment. Core C will alleviate the need for each of the investigators to establish the techniques in their individual laboratories, thereby increasing efficiency, reducing experimental variation, and providing economy of scale. The main advantages and innovation of Core C are to provide a dedicated, highly skillful, accessible and cost effective resource for animal models and tissue analysis. The proposed Core C has extensive experience in animal models for cardiovascular research focusing on various disease models, including thrombosis, atherosclerosis, vasculitis, stroke, cardiac hypertrophy, analysis and quantitation of mouse tissue and fluids specimens. The models and analysis have been well-established and published (see Biosketch of Core C leader). Building on the experience with animal models and tissue analysis, Core C has also established both spontaneous and pristane-induced SLE models (See Figures 6 and 7 in Research Plan). Core C will be led by Yunmei Wang PhD, who has extensive experience in murine models of vascular injury, thrombosis, atherosclerosis, stroke, and cardiac hypertrophy. In addition, Dr. Wang's over 25-years' research experience working with animals has prepared her well for any additional expertise and skills involving animals if needed for Core C. Core C will quickly adopt any innovative animal models once they become available and are determined appropriate for the purpose of the projects. Furthermore, Dr. Wang has collaborated with all project leaders of the PPG (see publication in Biosketch).