Physiology

项目摘要-生理学核心 该愿景研究核心(VRC)的总体目标是提供： ·获得个人R01奖励范围以外的资源 ·获得单一实验室范围以外的技术专长 ·员工培训，以消除高效翻译研究和协作的障碍 ·VRC实验室之间的协作计划 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发育障碍、青光眼和其他障碍，以及一系列尖端基础科学 首创精神。我们已经实现了四个资源模块，它们继续自然演变为 研究小组通力合作，为17名研究人员提供服务，获得了22个NEI R01奖。 生理学模块是VRC教员用于动物模型验证和疾病的一套强大的工具 分析：ERGS、OptoMotry行为测试和一系列眼部成像工具(OCT、美光)。我们的新产品 模块包括体内/体外双光子成像。 具体地说，生理学模块提供： ·三个UTAS ERG系统(两个在JMEC间隔室内) ·JMEC vivarum内的OptoMotry系统 ·两个微米级小鼠成像系统(一个在JMEC隔膜内) ·增加了一个新的Micron IV小鼠成像系统，具有高分辨率OCT、图像引导ERG 功能、裂隙灯和焦点激光伤害子模块，最近用该部的资金购置 和NEI的行政补充(P30EY014800-14S1) ·在JMEC隔核内用于运动和前庭表型的旋转棒 ·双光谱OCT系统(JMEC间隔室内的一个) ·一台布鲁克Ultima双光子显微镜(在5楼的核心) ·为所有这些系统提供技术支持

Project Summary-Physiology Core The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration · collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Physiology Module is a powerful set of tools that VRC faculty use for animal model validation and disease profiling: ERGs, OptoMotry behavioral testing, and a range of ocular imaging tools (OCT, Micron). Our new module includes in vivo / in vitro 2-photon imaging. Specifically the Physiology Module provides: · Three UTAS ERG systems (two within the JMEC vivarium) · An OptoMotry system within the JMEC vivarium · Two Micron mouse imaging systems (one within the JMEC vivarium) · An additional new Micron IV mouse imaging system with high resolution OCT, image-guided ERG function, slit lamp and focal laser injury submodules, recently acquired with funds from the Department of Ophthalmology and an Administrative supplement of the NEI (P30EY014800-14S1) · Rotarod for locomotor and vestibular phenotyping within the JMEC vivarium · Dual Spectralis OCT systems (one within the JMEC vivarium) · A Bruker Ultima 2-photon microscope (in core on floor 5) · Technical support for all these systems