Cell Biology

项目摘要-细胞生物学核心 该愿景研究核心(VRC)的总体目标是提供： ·获得个人R01奖励范围以外的资源 ·获得单一实验室范围以外的技术专长 ·员工培训，以消除高效翻译研究和协作的障碍 ·VRC实验室之间的协作计划 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发育障碍、青光眼和其他障碍，以及一系列尖端基础科学 首创精神。我们已经实现了四个资源模块，它们继续自然演变为 研究小组通力合作，为17名研究人员提供服务，获得了22个NEI R01奖。 细胞生物学模块源于莫兰眼科中心在小鼠遗传学方面的优势 对组织培养和图谱的需求。几乎每个JMEC实验室都需要高质量的资源来 冷冻切片、切片、基因分型、免疫细胞化学、定量聚合酶链式反应(QPCR)、细胞培养和 小鼠群体保存。一些JMEC实验室未来将需要诱导多能干细胞 (IPSC)的产生与视网膜器质分化。 具体而言，细胞生物学模块提供： ·通过两个专业维护的徕卡CM3050S系统提供高质量的冷冻手术资源 ·通过徕卡Paratome进行石蜡包埋和切片服务 ·聚合酶链式反应和基因分型服务 ·冷冻切割、免疫细胞化学、基因分型和定量聚合酶链式反应培训 ·通过获得装备良好和维护良好的生物安全二级(BSL2)细胞获得细胞培养资源 文化设施 ·小鼠系冷冻保存、ipsc生成和视网膜类器官的技术咨询 分化

Project Summary-Cell Biology Core The OVERALL aims of this Vision Research Core (VRC) are to provide: • access to resources outside the scope of individual R01 awards • access to technical expertise outside the scope a single laboratory • staff training to remove barriers to efficient translational research and collaboration • collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Cell Biology Module derives from the strengths in mouse genetics at the Moran Eye Center and the high demand for tissue culture and profiling. Almost every JMEC laboratory has need of high quality resources for cryosectioning, paratoming, genotyping, immunocytochemistry, quantitative PCR (qPCR), cell culturing, and mouse colony preservation. Some JMEC laboratories will have future need for induced pluripotent stem cell (iPSC) generation and retinal organoid differentiation. Specifically the Cell Biology module provides: • High-quality cryotomy resources through two professionally maintained Leica CM3050S systems • Paraffin embedding and section services via a Leica Paratome • PCR and genotyping services • Training of cryosectioning, immunocytochemistry, genotyping, and qPCR • Cell culture resources through access to a well-equipped and maintained biosafety level 2 (BSL2) cell culture facility • Technical consultation for mouse line cryopreservation, iPSC generation and retinal organoid differentiation