Proteomics

摘要：蛋白质组学共享资源 蛋白质组学SR(PROT)是一种最先进的设施，它将尖端的蛋白质组学研究带到LCCC 教职员工。它是一个完善的共享资源(SR)，为实验设计提供咨询， 为LCCC成员提供样品准备、质谱分析和广泛的数据分析。由一位导演 专家人员，SR是全方位的服务，允许有项目但几乎没有经验的用户了解范围 数据获取机会。SR还具有允许更有经验的用户访问的工具 昂贵的、维护得很好的质谱仪。核心员工和专家用户扩展了功能 通过创新的技术，如MIB/MS分析细胞范围的激酶活性。贡献包括 验证和表征与癌症相关的药物靶点，量化蛋白质组和信号网络 应对新兴疗法的变化，研究动态组的动态调节，描述如何 与基因表达变化无关的特定途径全球重塑癌症蛋白质组，以及 确定癌症中蛋白质相互作用网络是如何失调的。 仪器包括；最先进的质谱仪包括：Thermo Lumos、Thermo QExactive HF- X，Thermo Q Exactive Biophma，Thermo Q Exactive HF，Thermo Orbitrap Velos和Sciex 5800 MALDI/TOF/TOF。所有这些都是由LCCC部分购买或租赁的，其中4家是在过去4年内购买或租赁的。这个 购买和维护目前的PROT SR仪器超出了任何一个实验室的能力范围。 LCCC的投资使多个实验室有可能获得高端技术。Prot的成本 高级研究人员服务一般比可比的私人公司或其他学术机构低25%， LCCC会员可以获得额外的折扣，这使得该设施非常具有成本效益。LCCC要求 来自CCSG的135,301美元，占总运营预算的21%；服务请求使用量的48%是到 LCCC教职员工。

ABSTRACT: PROTEOMICS SHARED RESOURCE The Proteomics SR (PROT) is a state-of-the-art facility that brings cutting edge proteomics research to LCCC faculty. It is a well-established shared resource (SR) that provides consultation for the design of experiments, sample preparation, mass spectrometry (MS) and extensive data analysis for LCCC members. Directed by an expert staff, the SR is full service allowing users with projects but little experience to understand the range of data acquisition opportunities. The SR also has the instrumentation that allows more experienced users access to expensive, well maintained mass spectrometers. Core staff and expert users have extended the capabilities with innovative techniques such as the MIB/MS analysis of cell wide kinase activity. Contributions include validating and characterizing cancer-relevant drug targets, quantifying proteome and signaling network changes in response to emerging therapeutics, studying the dynamic regulation of the kinome, describing how specific pathways globally remodel cancer proteomes independent of changes in gene expression, and determining how protein interaction networks are dysregulated in cancer. Instrumentation includes; state-of-the-art mass spectrometers include: Thermo Lumos, Thermo QExactive HF- X, Thermo QExactive Biopharma, Thermo QExactive HF, Thermo Orbitrap Velos and Sciex 5800 MALDI/TOF/TOF. All were partially purchased or leased by LCCC including four within the last four years. The purchase and maintenance of the current PROT SR instruments are beyond the means of any individual lab. LCCC investments have made it possible for multiple labs to access high end technology. The cost of PROT SR services are generally lower by 25% than comparable private company or other academic institutions and LCCC members receive an additional discount making the facility quite cost effective. The LCCC is requesting $135,301 from the CCSG, which represents 21% of the total operating budget; 48% of the SR usage is by LCCC faculty.