Defining mechanisms of microbe-microbe interactions in chronic wound infection

项目摘要 金黄色葡萄球菌是世界范围内人类感染的主要原因。葡萄球菌调查 金黄色葡萄球菌的致病机制主要集中在单种感染和共感染微生物对金黄色葡萄球菌的影响。 金黄色葡萄球菌在感染过程中的生理学仍然研究不足，但至关重要。我最近的工作主要集中在 表征S.使用Next-在铜绿假单胞菌单一和合并感染期间观察金黄色葡萄球菌生理学 代测序技术。目前的应用程序逻辑上建立在这项工作，并侧重于 描述了S.金黄色葡萄球菌和慢性伤口感染中的共感染微生物。 本申请的总体目标是双重的：1）定义S. 金黄色葡萄球菌和共感染的微生物在感染（具体目标1）;和2）扩大这些发现， 确定微生物-微生物相互作用对S.人类感染中的金黄色葡萄球菌生理学（特异性 目标2）。这项工作很可能会产生重要的发现，有助于我们了解潜在的 慢性多微生物感染中微生物-微生物相互作用的机制。

Project Summary Staphylococcus aureus is a leading cause of human infection worldwide. Investigations into Staphylococcus aureus pathogenesis have focused on mono-species infections and the impact of co-infecting microbes on S. aureus physiology during infection remains understudied but critically important. My recent work has focused on characterizing S. aureus physiology during mono- and co-infection with Pseudomonas aeruginosa using next- generation sequencing techniques. The current application builds logically on this work and is focused on characterizing the interactions between S. aureus and co-infecting microbes within chronic wound infections. The overall goal of this application is two-fold: 1) to define the metabolic interactions that occur between S. aureus and co-infecting microbes during infection (Specific Aim 1); and 2) to expand on these findings to determine the impact of microbe-microbe interactions on S. aureus physiology within human infections (Specific Aim 2). This work is likely to yield important discoveries that will aid in our understanding of the underlying mechanisms of microbe-microbe interactions within chronic polymicrobial infections.