Caenorhabditis Genetics Center

项目概要/摘要-资源部分 小杆线虫遗传学中心（CGC）资源组件的目标是促进以下方面的研究： 小型后生动物秀丽隐杆线虫通过策展重要的、具有遗传特征的线虫种群 并应要求向研究人员和科学教育工作者分发。CGC是唯一的普通股 中心的策展和分发的C。优雅CGC目前拥有23，000种不同的菌株， 它们非常受欢迎：每年约有30，000种菌株被分发到数千个实验室， 大多数是发送给美国的用户组。应要求，通过在线 订购系统，并制定用户费用计划，以帮助支付成本和支持CGC活动。 所有地区的研究人员都为我们提供了他们产生的重要菌株。如果CGC没有 执行这种管理和分配服务，菌株共享将是非常低效和昂贵的; 填写申请的负担将由各个实验室承担，可能导致交换申请的延迟。 研究材料。此外，许多已发表的菌株的保存可能处于危险之中。这将是 这是一个巨大的损失，因为对这种模式生物的研究已经导致了对基本生物学的基本见解， 机制，包括程序性细胞死亡，轴突导向，以及RNA干扰的机制， 动物C. elegans还为癌症进展和神经退行性疾病的机制提供了见解。 这种线虫是阐明寄生线虫生物学的关键模型， 健康问题。除了促进研究进展外，CGC资源部分还提供了主要的成本- 节省到NIH作为一个整体，由NIH资助的个别研究实验室，以及所有其他实验室在 社区这些节省是通过减少冗余劳动力来实现的， 突变体组合容易获得，通过提供规模经济降低劳动力和其他成本 方法，而不是让单个实验室负责传播有用的菌株，保护菌株 通过在冗余站点进行管理，并促进使用这种相对较低的- 研究人员主要在高成本模型（如小鼠）中工作。

Project Summary/Abstract—Resource Component The objective of the Caenorhabditis Genetics Center (CGC) Resource Component is to promote research on the small metazoan Caenorhabditis elegans by curating important, genetically characterized nematode stocks and distributing them upon request to researchers and science educators. The CGC is the sole general stock center for the curation and distribution of C. elegans. The CGC currently houses 23,000 different strains, and they are immensely popular: ~30,000 strains are distributed to thousands of laboratories each year, and the majority are sent to user groups in the United States. Strains are distributed upon request through an on-line ordering system with a scheme of user fees in place to help defray costs and support CGC activities. Researchers in all locales supply us with important strains that they have generated. If the CGC were not performing this curation and distribution service, strain sharing would be extremely inefficient and costly; the burden of filling requests would be placed upon individual labs, likely leading to delays in the exchange of research materials. Moreover, the conservation of many published strains might be in jeopardy. This would be a great loss, because research in this model organism has led to fundamental insights into basic biological mechanisms, including programmed cell death, axonal guidance, and the mechanism of RNA interference in animals. C. elegans has also provided insights to mechanisms of cancer progression and neurodegenerative diseases, and this nematode serves as a key model for illuminating the biology of parasitic nematodes, a major health concern. In addition to enhancing research progress, the CGC Resource Component offers major cost- savings to the NIH as a whole, to individual research labs funded by NIH, and to all other labs in the community. These savings are achieved through decreasing redundant labor by making new mutations and mutant combinations readily available, decreasing labor and other costs by providing an economy-of-scale approach rather than having individual labs responsible for disseminating useful strains, safeguarding strains made with NIH grant support through curation in redundant sites, and facilitating the use of this relatively low- cost model by investigators working primarily in high-cost models such as mice.