Enable high-content live imaging in the study of constraint related morphome

项目摘要 脑发育是空间环境下最基本、最具影响力的生物行为 约束了解它们的生物物理过程和分子机制， 受益于新出现的类器官和其他3D组织模型。我们的目标是 描述这些3D组织中复杂细胞组织的形态学特征 通过先进的成像工具。 该建议的设备是一个高内容，共焦，现场成像系统，提供了一个 我们目前类器官分析的技术飞跃。通过这种技术的飞跃 设备将实现三大改进。1)3D深度成像允许追踪 组织形态和分化模式。2)共焦实时成像可以捕捉到 单个细胞的动态移位，提供时间的实验数据- 依赖理论模型3)高容量成像使扩展成为可能 实验总之，该成像系统将有助于我们目前的研究抓住新的 研究机会，并支持新的科学思想的发展， 脑类器官的形态学研究。

PROJECT SUMMARY Brain cerebral developments are fundamental and impactful bio-behaviors under spatial constraints. Understanding their biophysical process and molecular mechanisms can be benefited using the emergent organoids and other 3D tissue models. We aim to characterize the morphomics of the complex cell organizations in these 3D tissue models through advanced imaging tools. This proposed equipment is a high-content, confocal, live imaging system, providing a technological leap in our current organoid analysis. The technological leap through this equipment will enable three major enhancements. 1) 3D deep imaging allows tracing tissue morphology and differentiation patterns. 2) Confocal live imaging can capture the dynamic translocation of individual cells, providing experimental data for time- dependent theoretical modeling. 3) High-content imaging makes it possible to scale up the experiments. In summary, this imaging system will help our current study seize new research opportunities and support the development of novel scientific ideas in the morphomic study of brain organoids.