Genomics and Bioinformatics Core

项目摘要/摘要 下一代生物医学的发展推动了一场生物医学革命 测序(NGS)技术，使在 系统级别。现在，不是确定干扰一个基因的效果，而是所有基因都可以 同时进行评估。与其问转录因子如何与单个启动子结合 或增强子，可以在一次实验中确定该因子的所有结合位点。此外 为了观察复杂、异质组织中所有细胞的表达事件，我们可以 确定单个单元格级别的更改。利用这些方法涉及到 专业技术和分析技能的应用超出了大多数人的能力范围 实验室。考虑到这些问题，并需要在全基因组范围内应用尖端技术 对于与糖尿病、肥胖症和代谢性疾病相关的问题，我们有 建立了功能基因组学和生物信息学核心。此核心的目标是提供 向BADERC社区成员提供最先进的方法，以增强 并有助于更深入地了解代谢性疾病是如何发展的，以及它是如何 诊断和治疗。

PROJECT SUMMARY/ABSTRACT There has been a revolution in biomedicine fueled by the development of next generation sequencing (NGS) technologies which has made it possible to address biological questions at a systems level. Now, instead of determining the effect of perturbing one gene, all genes can be assessed simultaneously. Instead of asking how a transcription factor binds to a single promoter or enhancer, all binding sites for that factor can be determined in a single experiment. In addition to looking at expression events across all the cells within a complex, heterogenous tissue, we can determine changes at the single cell level. Taking advantage of these approaches involves the application of specialized technical and analytical skills beyond the reach of most individual laboratories. Mindful of these issues, and of the need to apply cutting edge genome-wide technology to questions of relevance to diabetes, obesity, and metabolic disease, we have established a Functional Genomics and Bioinformatics Core. The goals of this Core are to provide access to state-of-the-art methods to members of the BADERC community in order to enhance and facilitate a deeper understanding of how metabolic disease develops, and how it can be diagnosed and treated.