Monitor single-cell dynamics using optically computed phase microscopy in correlation with fluorescence characterization of intracellular properties

项目摘要 本研究的目的是开发一种综合复杂相位的多通道成像平台 基于光学计算的显微镜(CPM)和荧光显微镜(FM)。我们将使用成像平台来 将亚细胞的动态运动与细胞内的特性联系起来研究细胞的黏附，对研究细胞黏附具有重要意义 在细胞行为的许多方面。本研究对基础研究、临床前研究和临床研究具有重要意义。 技术发展。通过同时成像细胞运动和细胞器属性，CPM-FM使 相关生物分子特性中的细胞动力学研究。CPM-FM将对细胞黏附进行研究 更好地了解不同疾病的病理生理学，打开疾病治疗和治疗的新方法之门 早期诊断，导致开发需要与细胞特定相互作用的生物材料，并使患者和 临床医生。这项研究还将确定CPM作为一种成像技术的可行性，它允许无创、 在没有造影剂的情况下持续监测细胞活动。我们将通过以下途径实现这项研究的目标 开发CPM成像平台(AIM 1)，研究利用CPM进行高通量单细胞动态成像 (目标2)，并使用CPM研究粘附性与细胞存活率之间的相关性 显微镜(目标3)。该项目将为本科生提供一个进行研究的独特平台。 本科生研究助理将在细胞成像、图像处理和数据分析方面发挥主要作用。本科生 参与这项研究的研究助理接触到多学科的研究，这将为他们的职业生涯做好准备 在生物医学领域。

Project Summary The objective of this study is to develop a multi-modality imaging platform that integrates complex phase microscopy (CPM) based on optical computation and fluorescence microscopy (FM). We will use the imaging platform to correlate the sub-cellular dynamic motion and intracellular properties to study cell adhesion which plays an important role in many aspects of cell behavior. This study is significant for fundamental research, preclinical and clinical study, and technology development. By simultaneously imaging cell motion and organelle properties, CPM-FM enables the investigation of cell dynamics in correlation biomolecular characteristics. CPM-FM investigation of cell adhesion will result in better understanding of pathophysiology of different diseases, open the door to new methods for disease treatment and early diagnosis, lead to the development biomaterials that need specific interaction with cells, and benefit patients and clinicians. This study will also establish the feasibility of CPM as an imaging technology that allows non-invasive, and continuous monitoring of cell activities without contrast agents. We will achieve the objective of this study through developing the CPM imaging platform (Aim 1), investigating high-throughput single-cell dynamic imaging using CPM (Aim 2) and investigating the correlation between adhesive properties using CPM and cell viability using fluorescence microscopy (Aim 3). This project will provide a unique platform for undergraduate students to conduct research. Undergraduate research assistants will play major roles in cell imaging, image processing, and data analysis. Undergraduate research assistants participating in this study are exposed to multi-disciplinary research that will prepare them for a career in the fields of biomedicine.