Center for Epigenomics of the Mouse Brain Atlas (CEMBA)

摘要 了解小鼠大脑不同区域的确切细胞类型组成是一个基本步骤 当试图整合生理、行为、神经化学和分子数据时。目前虽然 存在于小鼠脑中的细胞类型的主要类别已经通过一些特定的 标记，这些类别中的不同亚型，以及它们的位置和连接性， 明白表观基因组特征如DNA甲基化（mC）和开放染色质是稳定的 在有丝分裂后细胞的整个生命周期中持续存在的修饰，定义了它们的细胞身份。开放 染色质以及甲基化模式是细胞类型特异性的，区分主要类型的细胞，即， 神经元和神经胶质，在啮齿动物和人类皮层，以及分化的神经元类型在小鼠大脑。 该中心的研究部分1提出了一个甲基化和开放染色质模式的目录 在整个小鼠大脑的单细胞水平上。对组合数据的分析将允许 发现细胞类型特异性调节区，其将允许产生转基因小鼠系， 病毒式的工具，将成为可用的社区。

Abstract Understanding the exact cell-type composition in the different regions of the mouse brain is a fundamental step when trying to integrate physiological, behavioral, neurochemical and molecular data. At present, although major categories of cell-types present in the mouse brain have been defined through a handful of specific markers, the different subtypes within these categories, as well as their location and connectivity are far from understood. Epigenomic signatures such as DNA methylation (mC) and open chromatin are stable modifications that persist in post-mitotic cells throughout their lifetime, defining their cellular identity. Open chromatin as well as methylation patterns are cell type specific, differentiating the major types of cells i.e., neurons and glia, in the rodent and human cortex, as well as differentiating neuronal types in mouse brain. Research Segment 1 of this center proposes to produce a catalog of methylation and open chromatin patterns at the single-cell level throughout the entire mouse brain. Analysis of the combined data will permit the discovery of cell-type specific regulatory regions that will allow the production of transgenic mouse lines and viral tools that will become available to the community.