Anatomical and Functional Interrogation of Parallel Visual Pathways from Eye to Brain
从眼睛到大脑的平行视觉通路的解剖学和功能询问
基本信息
- 批准号:10412937
- 负责人:
- 金额:$ 46.66万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-05-01 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:AnatomyApicalArchitectureAwardBehaviorBlindnessBrainCalciumCellsCharacteristicsDendritesDiseaseDorsalEquilibriumEyeGoalsHumanImageLabelLateral Geniculate BodyLeadMorphologyMusNeuronsOutputPathway interactionsPatternPerceptionPopulationPrimatesProcessPropertyRabiesRecurrenceResearchRetinaRetinal Ganglion CellsShapesSpeedSynapsesTestingThalamic structureTranslatingV1 neuronViralVisualVisual PathwaysVisual PerceptionVisual system structureWhole-Cell RecordingsWorkarea striatacell typedesigner receptors exclusively activated by designer drugsexperimental studyextracellularinnovationinsightmulti-electrode arraysneural circuitnoveloptogeneticsrabies viral tracingresponsesight restorationtherapy designtherapy developmenttwo-photonvisual informationvisual processvisual processing
项目摘要
Project Summary/Abstract
The long-term goal of this proposal is to characterize the connectivity of specific circuits in the mouse visual
system whose gross architecture is highly conserved across species from mouse to primate. The objective is
to identify the neural circuits that transmit visual information from the retina, through different regions of the
dorsal lateral geniculate nucleus (dLGN), to specific populations of neurons in the primary visual cortex (V1)
that contribute to visual perception and behavior. The central hypotheses of this proposal are: (1) that neurons
in the shell and core of the dLGN receive different types of visual information from the retina and, therefore,
can transmit different types of visual information to V1 neurons; (2) The balance of core and shell input that V1
neurons receive influences their tuning properties. The experiments outlined in this proposal will test these
hypotheses by pursuing three specific aims: (1) Identifying and characterizing the retinal ganglion cell (RGC)
types that provide input to neurons in the core of the dLGN, as well as the neurons that those dLGN neurons
contact in V1; (2) Determining whether genetically-identified neurons in V1 receive input from unique patterns
of RGCs; and (3) Determining how input from neurons in the core of the dLGN influence tuning properties in
genetically-identified populations of V1 neurons. This proposal is technologically innovative; it will use novel
mouse lines as well as a combination of rabies circuit tracing, whole-cell recording, optogenetic stimulation,
chemogenetic silencing, and two-photon calcium imaging to accomplish its aims. The proposed research will
yield significant findings that will provide considerable insight into how information is encoded, processed, and
ultimately transmitted throughout the mouse visual pathway. These findings are of utmost importance as the
computations performed in these pathways generate a representation of the visual scene and ultimately make
characteristic contributions to perception and behavior. The proposed research will also help determine the
extent to which visual processing in the mouse visual system does, or does not, mimic visual processing in the
primate visual system. Understanding the extent to which computations performed in the visual pathway
overlap in mouse and primate visual systems is critical for determining how research done in in the mouse
visual system translates to the primate — and therefore human — visual system. Indeed, to successfully
develop strategies to restore sight across a wide range of afflictions of the visual system, it is critical to first
understand how the visual system gives rise to our sense of the world around us. The work proposed here will
unequivocally move us closer to this goal.
项目摘要/摘要
这项提议的长期目标是表征鼠标视觉中特定电路的连接
其总体结构在从老鼠到灵长类的物种中高度保守的系统。我们的目标是
为了识别从视网膜传递视觉信息的神经回路,通过视网膜的不同区域
背外侧膝状核(DLGN),至初级视皮层(V1)的特定神经元群
对视觉感知和行为有贡献。这一提议的中心假设是:(1)神经元
在dLGN的外壳和核心中从视网膜接收不同类型的视觉信息,因此,
可将不同类型的视觉信息传递给V1神经元;(2)V1核壳输入的平衡
神经元受到影响,它们的调谐特性。这项提案中概述的实验将测试这些
通过追求三个特定目标的假设:(1)鉴定和鉴定视网膜神经节细胞(RGC)
为dLGN核心的神经元提供输入的类型,以及这些dLGN神经元
V1中的接触;(2)确定V1中遗传识别的神经元是否接受来自独特模式的输入
以及(3)确定来自dLGN核心神经元的输入如何影响
遗传识别的V1神经元群体。这项提议在技术上是创新的;它将使用新颖的
以及狂犬病电路追踪、全细胞记录、光遗传刺激、
化学发生沉默和双光子钙成像,以实现其目标。拟议的研究将
产生重要的发现,这些发现将为信息如何编码、处理和
最终通过老鼠的视觉通路传播。这些发现至关重要,因为
在这些路径中执行的计算生成视觉场景的表示,并最终使
对感知和行为的特性贡献。拟议的研究还将有助于确定
鼠标视觉系统中的视觉处理模仿或不模仿
灵长类视觉系统。了解计算在视觉通路中执行的程度
小鼠和灵长类动物视觉系统的重叠对于决定如何在小鼠身上进行研究至关重要
视觉系统转化为灵长类动物--因此也就是人类--视觉系统。事实上,要想成功
制定恢复视力的策略,克服各种视觉系统疾病,关键是首先
理解视觉系统如何产生我们对周围世界的感觉。这里提出的工作将
毫不含糊地让我们更接近这一目标。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('EDWARD M CALLAWAY', 18)}}的其他基金
Genetic access to cortical cell types with epigenetic assays and high-throughput, low-cost enhancer screening
通过表观遗传分析和高通量、低成本增强子筛选对皮质细胞类型进行遗传获取
- 批准号:
10025622 - 财政年份:2020
- 资助金额:
$ 46.66万 - 项目类别:
Genetic access to cortical cell types with epigenetic assays and high-throughput, low-cost enhancer screening
通过表观遗传分析和高通量、低成本增强子筛选对皮质细胞类型进行遗传获取
- 批准号:
10462798 - 财政年份:2020
- 资助金额:
$ 46.66万 - 项目类别:
Genetic access to cortical cell types with epigenetic assays and high-throughput, low-cost enhancer screening
通过表观遗传分析和高通量、低成本增强子筛选对皮质细胞类型进行遗传获取
- 批准号:
10237360 - 财政年份:2020
- 资助金额:
$ 46.66万 - 项目类别:
Center for Epigenomics of the Mouse Brain Atlas (CEMBA)
小鼠大脑图谱表观基因组学中心 (CEMBA)
- 批准号:
9568015 - 财政年份:2017
- 资助金额:
$ 46.66万 - 项目类别:
Methodologically-Integrated Approaches Linking Cell Types to Neural Circuits and Function
将细胞类型与神经回路和功能联系起来的方法论集成方法
- 批准号:
9459190 - 财政年份:2017
- 资助金额:
$ 46.66万 - 项目类别:
Center for Epigenomics of the Mouse Brain Atlas (CEMBA)
小鼠大脑图谱表观基因组学中心 (CEMBA)
- 批准号:
9416014 - 财政年份:2017
- 资助金额:
$ 46.66万 - 项目类别:
Center for Epigenomics of the Mouse Brain Atlas (CEMBA)
小鼠大脑图谱表观基因组学中心 (CEMBA)
- 批准号:
10252523 - 财政年份:2017
- 资助金额:
$ 46.66万 - 项目类别:
Resources for Studying Neural Circuit Structure and Function with G-Deleted Rabies Viruses
研究 G 缺失狂犬病病毒神经回路结构和功能的资源
- 批准号:
9526570 - 财政年份:2015
- 资助金额:
$ 46.66万 - 项目类别:
Robust trans-synaptic labeling technologies for cell type-specific quantitation of synaptic connectivity
强大的跨突触标记技术,用于突触连接的细胞类型特异性定量
- 批准号:
8935699 - 财政年份:2015
- 资助金额:
$ 46.66万 - 项目类别:
Resources for Studying Neural Circuit Structure and Function with G-Deleted Rabies Viruses
研究 G 缺失狂犬病病毒神经回路结构和功能的资源
- 批准号:
9130302 - 财政年份:2015
- 资助金额:
$ 46.66万 - 项目类别:
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