Kansas Center for Metabolism and Obesity REsearch (KC-MORE) - Metabolism Core

代谢核心：项目总结 代谢在肥胖的发展和肥胖的病理后果中起着基础性的作用 导致慢性病的原因能量平衡失调和底物储存或氧化受损 是肥胖和代谢疾病的基本特征。代谢失调是由两个驱动 细胞线粒体功能的改变和全身代谢稳态的破坏。因此，我们认为， 将细胞线粒体能量学与全身体内底物代谢联系起来的代谢研究 能量消耗对于理解代谢功能障碍、肥胖和 肥胖相关的疾病。代谢（MET）核心将提供堪萨斯代谢中心和 肥胖研究（KC-MORE）的专业知识，方法和设备的研究"代谢"，从 从细胞到整个身体。MET核心的核心任务将是开发一个新的中央MET核心设施， 临床前模型（啮齿动物），提供了一系列关键测量，允许进行全面的代谢 表型分析这些方法将包括专门知识和设备，以量化体内的真实的时间测量。 能量代谢、底物代谢、食物摄入调节和葡萄糖/胰岛素耐量测试沿着 在临床前啮齿动物模型（大鼠和小鼠）中进行身体组成的非侵入性测量。第二核心 MET核心的一个特点是为研究人员提供量化细胞中线粒体能量学的资源 以及啮齿动物和人类样本的组织。最后，MET核心还将具有执行 代谢同位素方法（同位素标记的葡萄糖、脂肪酸等）用于人类或啮齿动物模型 （体内或离体）来量化总体代谢通量。总而言之，MET核心服务的组织方式是： 为研究临床前啮齿动物模型系统代谢的研究人员提供最先进的核心服务 并涉及用于细胞，组织， 啮齿动物和人类参与者。MET核心的设计也在很大程度上与人类的能力相平行。 能量平衡核心，以便KC-MORE的科学家可以在啮齿动物和 人类

METABOLISM CORE: PROJECT SUMMARY Metabolism plays a fundamental role in the development of obesity and the pathological consequences of obesity that lead to chronic disease. Dysregulation of energy balance and impaired storage or oxidation of substrates are fundamental features of developing obesity and metabolic disease. Metabolic dysregulation is driven by both alterations in cellular mitochondrial function and disruptions in whole body metabolic homeostasis. Therefore, metabolic research that connects cellular mitochondrial energetics to whole body in vivo substrate metabolism and energy expenditure is critical for understanding mechanisms underlying metabolic dysfunction, obesity, and obesity-related disease states. The Metabolism (MET) core will provide the Kansas Center for Metabolism and Obesity REsearch (KC-MORE) with expertise, methodologies, and equipment for the study of “metabolism” from the cell to the whole body. Core missions of the MET core will be to develop a new central MET core facility for pre-clinical Models (rodents) that provides a host of key measurements, allowing for thorough metabolic phenotyping. These approaches will include expertise and equipment to quantify real time measures of in-vivo energy metabolism, substrate metabolism, food intake regulation, and glucose/insulin tolerance testing along with non-invasive measures of body composition in pre-clinical rodent models (rat and mice). A second core feature of the MET core will be to provide resources to investigators to quantify mitochondrial energetics in cells and tissues from both rodent and human samples. Finally, the MET core will also have the capability to perform metabolic isotope methodologies (isotopic labeled glucose, fatty acids, etc.) for use in humans or rodent models (in-vivo or ex-vivo) to quantify overall metabolic flux. In summary, the MET core services will be organized to provide state-of-the art core services to investigators studying metabolism in pre-clinical rodent model systems and to translational measures of mitochondrial energetics and stable metabolic isotopes for use in cells, tissues, rodents, and human participants. The MET core is also designed to largely parallel the capabilities of the Human Energy Balance core so that scientists in the KC-MORE can conduct translational studies in both rodents and humans.