Resource Center for Human Temporal Bone Research, Innovation, and Education

摘要 对人类颞骨的研究已经证明是非常强大的， 我们知道耳朵的解剖学和病理学。然而，耳病理学研究的传播一直是 受到影响研究链每一步（采购、加工、分销和 传播）。该项目的主要目标是推广人类颞骨的研究用途 通过建立我们的实验室作为颞骨专业知识的国家资源， NIDCD将建立的合作网络的一部分，为听觉和前庭研究服务 社区.这些目标将通过四个具体目标来实现。在具体目标1中，我们将建立一个 提供优质颞骨组织的加工和配送服务。我们将利用我们的高 采购能力、藏品数字化以及实施结构化方法的技术经验 用于优先处理和处理具有特殊科学意义的人类颞骨，以满足研究 科学界的需要。在特定目标2中，我们将优化人颞骨制备 降低加工成本和时间并提高保留、保存和提取蛋白质的能力的技术 DNA/RNA我们将研究提高定影速度和质量的技术， 包埋、脱钙、切片和可视化。在具体目标3中，我们将提供技术指导 为科学和临床界提供关于人类颞骨的处理和使用的信息。创新混合 将采用教育模块，所有材料、培训和协议将免费分发给 增加训练有素的技术人员和研究人员的数量。在具体目标4中，我们将提供外展服务， 科学和临床界传播和促进人类颞骨在研究中的使用。 这将在重大科学会议上亲自实现，并实际使用多种多媒体资源。 我们的多学科团队经验丰富的研究人员具有必要的知识和技能， 充足的资源（大量的档案收藏，高质量颞骨的高采购能力， 技术熟练的技术人员团队，内部分发服务，发送生物标本），与 卓越的成像和组织处理中心，以及创新的教育和视听服务， 提供必要的手段，传播和采取颞骨研究到一个新的水平。

ABSTRACT The study of the human temporal bone has proven to be incredibly powerful and is responsible for much of what we know about the anatomy and pathology of the ear. However, propagation of otopathology research has been hampered by barriers that affect every step of the research chain (procurement, processing, distribution, and dissemination). The major goals of this project are to propagate the use of human temporal bones for research and training through the establishment of our lab as national resource for temporal bone expertise and to become part of the collaborative network to be established by the NIDCD to serve the auditory and vestibular research communities. These goals will be achieved through 4 specific aims. In Specific Aim 1, we will establish a core service for processing and distribution of high-quality temporal bone tissue. We will leverage our high procurement capacity, digitization of our collection, and technical experience to implement a structured approach for prioritizing and processing human temporal bones that are of special scientific interest to meet research needs of the scientific community. In Specific Aim 2, we will optimize human temporal bone preparation techniques to reduce processing costs and time and improve the capacity to retain, preserve, and extract proteins and DNA/RNA. We will investigate technologies for improving the speed and quality achieved during fixation, embedding, decalcification, sectioning, and visualization. In Specific Aim 3, we will provide technical instruction on the processing and use of human temporal bones for the scientific and clinical communities. Innovative hybrid educational modules will be employed, and all materials, training, and protocols will be freely disseminated to increase the number of well-trained technicians and researchers. In Specific Aim 4, we will provide outreach to the scientific and clinical communities to disseminate and promote the use of human temporal bones in research. This will be achieved in-person at major scientific meetings and virtually using multiple multimedia resources. Our multidisciplinary team of experienced investigators has the necessary knowledge and skills aligned with ample resources (large archival collection, high procurement capacity of high-quality temporal bones, highly skilled team of technicians, internal distribution services to send biological specimens), collaborations with centers of excellence for imaging and tissue processing, and innovative educational and audiovisual services to provide the necessary means to propagate and take temporal bone research to the next level.