Conformational Energetics and Heterogeneity to Reveal Gating Mechanisms of TRPV and TRPM Ion Channels

项目摘要/摘要 这项要求行政补充以促进多样性的请求将使特蕾莎·M·斯旺森博士能够作为一名 戈登实验室的博士后学者。她将在母公司R35拨款范围内从事TRPM2项目的工作。 该项目研究了钙离子和ADP核糖驱动构象的分子机制。 TRPM2通道中的重排。斯旺森博士将在实验中直接跟随戈登博士进行训练 所需方法：使用稳态和时间分辨荧光法进行过渡金属离子荧光检测 测量；使用琥珀密码子抑制引入非天然氨基酸；以及膜片钳 荧光测定法。这个项目的工作将为Swanson博士提供极好的培训，以促进她的职业生涯。她会的 还参与职业发展机会和计划，以增强生物医学的多样性 员工队伍，如培训计划中所述。

Project Summary/Abstract This request for an administrative supplement to promote diversity will allow Dr. Teresa M Swanson to train as a postdoctoral scholar in the Gordon lab. She will work on the TRPM2 project in the scope of the parent R35 grant. This project addresses the molecular mechanisms governing Ca2+- and ADP Ribose-driven conformational rearrangements in TRPM2 channels. Dr. Swanson will train directly under Dr. Gordon in the experimental methods required: transition metal ion FRET using both steady-state and time-resolved fluorescence measurements; using amber codon suppression to introduce unnatural amino acids; and patch-clamp fluorometry. Work on this project will provide Dr. Swanson with excellent training to advance her career. She will also participate in career development opportunities and programs to enhance diversity of the biomedical workforce, as described in the training plan.