Improving The Scripps Research Institute BSL3 Capabilities to Combat Viruses of Pandemic Concern

项目摘要/摘要 应用程序的目标是提高斯克里普斯研究所BSL3对抗病毒的能力 大流行问题“是对斯克里普斯研究所(TSRI)目前的ABSL3和BSL3进行更新和升级 设施。这将增强TSRI的研究能力，以对抗具有大流行潜力的RNA病毒。TSRI拥有 约2,000 SF专门用于ABSL3，约1,500 SF专门用于BSL3。目前，这些设施支持研究 来自八个TSRI教员的项目，他们的专业领域结合在一起，涵盖了大流行RNA病毒的研究 潜在的包括布尼亚病毒科、冠状病毒科、黄病毒科、丝状病毒科和冠状病毒科。此外， 这些设施正在支持几个由制药公司赞助的项目。具体的研究有 旨在开发针对这些RNA的抗病毒药物和免疫疗法的强大翻译成分 病毒。位于分子生物学大楼(MBB)的TSRI ABSL3和BSL3套房一直在运行 自2004年最后一次翻修以来，全天候服务。现有的机械系统被保留了很久 ASHREA设备预期寿命，加利福尼亚州拉荷亚。沿海气候。此外，最初的设计来自 1985-87年导致ABSL3中的~160 SF和BSL3中的~400 SF未得到充分利用。要纠正这些问题 尽管存在不足，但TSRI已与FPBA Architects&Tk1sc机械工程师一起完成了概念设计。这 计划包括更换ABSL3和BSL3空间老化的暖通空调系统，以增加气流能力 N+1冗余并更新ABSL3的HEPA过滤系统。此外，该项目还将使 通过增加容纳能力(ABSL3)、创建选择药剂隔离室(BSL3)、 安装新的灭菌器(ABSL3)。目前的ABSL3/BSL3设施缺乏成像、细胞分选和单一 细胞分析能力。为了弥补这些不足，我们建议在设备中增加EVOSTM成像 系统、SH800S细胞分选机和Chromium IX系统。这些改进将有助于解决 TSRI教职员工对ABSL3/BSL3空间、时间和改进的功能能力的需求不断增加，同时 确保工厂日常运营期间的最高水平的安全。此外，建议开展的研究活动 根据大流行抗病毒计划(APP)U19AI171443 AViDD赠款申请，将大大受益 根据对现有TSRI ABSL3和BSL3设施的拟议改进。该项目将得到管理 由一支完善的、高素质的团队，包括设施建设和工程部门的领导， 机构动物计划和资源、EHS和生物安全、执行副总裁办公室和调查人员 直接参与ABSL3/BSL3研究活动。此外，该应用程序还得到了 TSRI领导层。

Project Summary/Abstract The goal of the application “Improving The Scripps Research Institute BSL3 Capabilities To Combat Viruses Of Pandemic Concern” is to renovate and upgrade The Scripps Research Institute (TSRI) current ABSL3 and BSL3 facilities. This will enhance TSRI research capabilities to combat RNA viruses of pandemic potential. TSRI has ~ 2,000 sf dedicated to ABSL3 and ~ 1,500 sf dedicated to BSL3. Currently, these facilities support research projects from eight TSRI faculty whose combined areas of expertise cover research on RNA viruses of pandemic potential, including Bunyavirales, Coronaviridae, Flaviviridae, Filoviridae and Togaviridae. In addition, the facilities are supporting several projects sponsored by pharmaceutical companies. The specific studies have strong translational components aimed at developing antiviral drugs and immunotherapeutics against these RNA viruses. TSRI ABSL3 and BSL3 suites, located in the Molecular Biology Building (MBB), have been operating 24/7 since the last renovations in 2004. The existing mechanical systems have been maintained beyond ASHREA equipment life expectancy for the La Jolla, Ca. coastal climate. In addition, the original design from 1985-87 has resulted in underutilization of ~160 sf in the ABSL3 and ~400 sf in the BSL3. To correct these deficiencies, TSRI has completed a conceptual design with FPBA Architects & tk1sc Mechanical Engineers. This plan includes replacing the aging HVAC systems of the ABSL3 and BSL3 space to increase airflow capacity with N+1 redundancy and update the HEPA filtration system of the ABSL3. In addition, the project will modernize the biocontainment suites by increasing holding capacity (ABSL3), creating a select agent isolation room (BSL3), and installing a new sterilizer (ABSL3). The current ABSL3/BSL3 facilities lack imaging, cell sorting, and single cell analysis capabilities. To remedy these deficiencies, we propose adding to the facility the EVOSTM Imaging System, the SH800S Cell Sorter and the Chromium iX system. These improvements will help to address the increasing demands by TSRI faculty for ABSL3/BSL3 space, time and improved functional capabilities, while ensuring the highest level of safety during daily operations of the facility. Moreover, research activities proposed under the Antiviral Program for Pandemics (APP) U19AI171443 AViDD Grant Application, will greatly benefit from the proposed improvements to the current TSRI ABSL3 and BSL3 facilities. The project will be managed by a well-integrated and highly qualified team, including leaders from Facilities Construction and Engineering, the Institutional Animal Program and Resources, EHS and Biosafety, the Executive VP Office, and investigators directly involved in ABSL3/BSL3 research activities. In addition, the application has the unwavering support of the TSRI leadership.