Core C: Cell and Gene Vector Core

核心C摘要 细胞和基因载体核心(核心C)将为 通过构建、繁殖和规模化生产腺病毒、慢病毒和 腺相关病毒(AAV)库存，这是所有项目都需要的。利用公司的丰富经验和专业知识 核心导演和内部制定的病毒载体生产协议，核心将提供 为支持项目提供一致和可重复的服务。此核心还将包含培养的专业知识 用于体外机械工作的心肌细胞，包括每周一次的新生大鼠心肌细胞制备和 需要时分离成年小鼠心肌细胞。核心的另一个好处是它提供了一个 教授他人组织培养和病毒生产技术的资源。这些基本的集中支持 所有项目使用的服务将消除病毒和肌细胞制剂的可变性，并将在 识别新的信号通路、器官内沟通通路和性别依赖机制 导致心脏损伤后的心力衰竭和心肾综合征，并确定潜在的交叉 制定创新的修复治疗策略的机制。

Core C SUMMARY The Cell and Gene Vector Core (Core C) will provide an essential centralized support service to the investigators on the grant by constructing, propagating and large-scale production of adenoviral, lentiviral and adeno-associated viral (AAV) stocks, which all Projects will need. Utilizing the vast experience and expertise of the Core Director's, and in-house established protocols for the viral vector productions, the Core will provide a consistent and reproducible service in support of the projects. This Core will also house expertise for cultured myocytes for in vitro mechanistic work including weekly neonatal rat ventricular myocyte preparations and isolation of adult murine cardiomyocytes when needed. An additional benefit of the Core is that it provides a resource for teaching others tissue culture and viral production techniques. These essential centralized support services, used by all Projects, will eliminate variability in virus and myocyte preparations and will be pivotal in identifying novel signaling pathways, intra-organ communication pathways and sex-dependent mechanisms that contribute to heart failure and cardiorenal syndrome after cardiac injury and identify potential intersecting mechanisms to develop innovative therapeutic strategies for repair.