HIPC U19 Adaptive Immunophenotyping Core

总结/摘要 适应性表型核心将提供先进的实验室方法，以检查全球和抗原- 为重债穷国中心生成的样本的特异性免疫反应， 呼吸道病毒感染的风险"。该核心将服务于两个研究项目， 从临床中心收集样本，并与数据管理部门共享高质量的细胞图谱， 分析核心。适应性表型核心有两个具体目标。在具体目标1中， Phenotyping Core将使用光谱流式细胞术生成骨髓细胞的高维分析数据， 外周血、鼻和痰样品中的B细胞和T细胞。在具体目标2中，自适应 Phenotyping Core将使用标准化工作流程分析外周血中的抗原特异性T细胞 包括基于激活诱导标记物（AIM）的测定、基于抗体的条形码和光谱流 细胞仪待检测的抗原特异性T细胞取决于研究项目1的项目 专注于过敏原特异性T细胞，研究项目2专注于cit-antigen-specific T细胞， 自身反应性T细胞参与类风湿性关节炎的发病机制。重要的是，这些抗原中的每一种- 特异性T细胞群将与呼吸道病毒特异性T细胞并行分析。自适应 表型分析核心将与每个项目和核心负责人密切合作。

SUMMARY/ABSTRACT The Adaptive Phenotyping Core will provide advanced laboratory approaches to examine global and antigen- specific immune responses on samples generated for the HIPC Center entitled “Systems immunology profiling of respiratory viral infections in vulnerable populations”. This core will serve both Research Projects, receiving samples from the Clinical Core and sharing high quality cellular profiles with the Data Management and Analysis Core. The Adaptive Phenotyping Core has two Specific Aims. In Specific Aim 1, the Adaptive Phenotyping Core will use spectral flow cytometry to generate high-dimensional profiling data on myeloid cells, B cells, and T cells in peripheral blood, nasal, and sputum samples. In Specific Aim 2, the Adaptive Phenotyping Core will profile antigen-specific T cells in peripheral blood using a standardized workflow including an activation-induced markers (AIM)-based assay, antibody-based barcoding, and spectral flow cytometry. The antigen-specific T cells to be examined is dependent on the project with Research Project 1 focused on allergen-specific T cells and Research Project 2 focused on cit-antigen-specific T cells, autoreactive T cells implicated in the pathogenesis of rheumatoid arthritis. Importantly, each of these antigen- specific T cell populations will be analyzed side-by-side with respiratory virus-specific T cells. The Adaptive Phenotyping Core will work closely with each of the Project and Core Leads.