Chemical Synthesis Core C

核心C化学合成 核心负责人：阿德里安·明纳德 摘要 化学合成核心C支持化学发现和基准实验的方式， 不能通过分析天然细菌产物来实现。而病原体中的天然化合物 分枝杆菌通常限于微克产量，化学合成可以提供许多毫克， 可持续提供大规模实验。而天然产物通常显示出许多几乎共洗脱 分子变体，合成可以产生单一的纯化合物，用于基准天然化合物， 作为严格确认新发现的化学结构的标准。合成类似物， 确定的化学变化支持结构-活性关系的确定。核心C位于 格罗宁根大学Stratingh研究所，世界领先的实验室合成化学中心 专门研究化学合成的对映体特异性催化方法。因此，核心C具有独特的 生产具有多个手性中心的细菌代谢物的专业知识，这些手性中心定义了复杂的支链 结构，如二真菌蜡酸苯硫代蜡醇酯、磺基糖脂、分枝菌酸、酚糖脂和其他 脂质。核心C将产生毫克数量的分枝杆菌代谢物，以支持项目1和3。在 此外，核心将提供现有的和新的化合物，以产生一套全面的 用于在分析和绘图核心B中对分枝杆菌脂质进行基准测试的标准。

Core C Chemical Synthesis Core Leader: Adriaan Minnaard ABSTRACT The Chemical Synthesis Core C supports chemical discovery and benchmarking experiments in ways that cannot be achieved by analysis natural bacterial products. Whereas natural compounds from pathogenic mycobacteria are generally limited to microgram yields, chemical synthesis can provide many milligrams to durably supply large-scale experiments. Whereas natural products typically show many nearly co-eluting molecular variants, synthesis can produce a single pure compound for benchmarking natural compounds and as a standard for rigorously confirming newly discovered chemical structures. Synthesis of analogs with defined chemical variations supports determination of structure-activity relationships. Core C is housed at the Stratingh Institute at the University of Groningen, a world leading center for synthetic chemistry in a laboratory that specializes in enantiospecific catalytic methods of chemical synthesis. Thus, Core C has a unique expertise in production of bacterial metabolites with multiple chiral centers that define complex branched chain structures, such as phthiocerol dimycocerosate, sulfoglycolipids, mycolic acid, phenolic glycolipid and other lipids. Core C will produce milligram quantities of mycobacterial metabolites to support Projects 1 and 3. In addition, the Core will provide both existing and new compounds to generate a comprehensive set of standards for benchmarking mycobacterial lipids in the Profiling and Mapping Core B.