Research Supplements to Promote Diversity in Vascular Graft Research

摘要 本申请的父授权书为R01HL159427，标题为 螺旋超细纤维“。父母资助的目标是设计一种无细胞的可降解移植物，宿主可以 变成一条自体的血管管道。该基金的一个主要目的是研究宿主重塑。 绵羊颈动脉模型中的长纤维移植物。我们在衰老的绵羊身上进行了五次植入。 我们了解到，移植物需要更坚固，以用于临床翻译。这方面的研究重点是 补充应用是消除纤维堆积和增加纤维在结合处熔融的手段 血管移植物更健壮。此外，家长赠款的执行主任Iwijn de Vlaminck博士告知 绵羊单细胞RNA测序的最新进展。这将极大地丰富信息。 我们是从我们的绵羊研究中收集的。因此，本副刊的研究方面将集中在以下几个方面 未来3年的具体目标： 1.消除纤维堆积，诱导移植物连接处纤维融合。 2.在绵羊研究中，使用单个细胞识别填充移植物的细胞的类型和潜在来源 细胞RNA测序。 电写血管移植物的设计和宿主重塑的研究是亲本的关键方面 格兰特。然而，无论是对纤维堆积和融合的研究，还是对绵羊移植物的rna测序的研究都没有。 在父母拨款中提出的。我们认识到这些是项目启动后研究的重要内容。 这项补充申请中提出的研究没有被初始审查小组删除。哈雷 韦尔奇是一名非洲裔美国博士生，他对血管移植项目非常热情。这 补充将增加少数族裔学生对血管移植研究的参与，并为哈雷做好准备 在学术研究方面的未来。因此，这一补充要求符合多样性的精神 副刊。

Abstract The parent grant of this application is R01HL159427 entitled “Elastic, Degradable Vascular Grafts with Helical Microfibers”. The goal of the parent grant is to design a cell-free degradable graft that the host can transform into an autologous vascular conduit. A major aim of the grant is to investigate host remodeling of long microfibrous grafts in a sheep carotid artery model. We performed five implantations in senescent sheep. We learned that the graft needed to be more robust for clinical translation. The research focus of this supplement application is to eliminate fiber stacking and increase fiber fusion at the junction as means to make the vascular graft more robust. Moreover, the Co-I of the parent grant, Dr. Iwijn De Vlaminck, informed the team of a recent advancement on single cell RNA sequencing in sheep. This will greatly enrich the information we gather from our sheep study. Therefore, the research aspect of this supplement will focus on the following specific aims in the next 3 years: 1. Eliminate fiber stacking and induce fiber fusion at the junctions in the graft. 2. Identify the type and potential origin of the cells populating the grafts in the sheep study using single cell RNA sequencing. The design of electrowritten vascular grafts and the study of host remodeling are key aspects of the parent grant. However, neither the study of fiber stacking and fusion, nor RNA sequencing of the sheep graft is proposed in the parent grant. We recognize these as important elements of the study after the project initiation. The research proposed in this supplemental application was not deleted by the initial review group. Halle Welch, a first year African American Ph.D. student, is very passionate about the vascular graft project. This supplement will increase the participation of minority students in vascular graft research and prepare Halle well for a future in academic research. Thus, this supplement request is consistent with the spirit of a diversity supplement.