Exosomal miRNA in neuron to astroglial communication in the CNS

总结 这一多样性补充旨在提供一个机会，拉希德阿兰泽谁满足国家卫生研究院 多样性候选人标准，在我的实验室进行学士后研究。在这个项目中，Rashed将 表征神经元外泌体从索马或轴突终末的分泌以及随后的 神经元外泌体内化为星形胶质细胞。虽然管腔内囊泡（ILV），前体 分泌的外泌体，主要定位于培养神经元的体树突区室，这是未知的 这些ILV是否优先从体内神经元索马或轴突分泌，考虑到囊泡 神经递质的分泌主要从轴突末梢释放。利用坐骨神经 注射模型以选择性标记脊髓运动神经元（SMN）外泌体和玻璃体内注射以选择性 标记视网膜神经节细胞（RGC），Rashed将专门研究和比较神经元外泌体是否 从体树突或轴突终末隔室差异释放，这些 外泌体也不同地被周围的星形胶质细胞摄取。 了解外泌体分泌的主要位置从这样一个极化的细胞类型，如神经元 将对这些外来体的潜在功能提供重要的见解。通过实施该项目， 他有机会了解如何看到一个项目，从概念到完成，学习各种注射 技术，处理科学实验中总是需要的故障排除和优化， 学习分析图像和数据的最佳方法。总的来说，这一多样性补充奖将大大准备 急着发展他的研究事业。

Summary This diversity supplement aims to provide an opportunity for Rashed Alananzeh who meets the NIH diversity candidate criteria, to carry out a post-Baccalaureate study in my lab. In this project, Rashed will characterize the secretion of neuronal exosomes from either soma or axon terminals and the subsequent internalization of neuronal exosomes into astrocytes. Although intraluminal vesicles (ILVs), precursors for secreted exosomes, are largely localized in the somatodendritic compartment of cultured neurons, it is unknown whether these ILVs are preferentially secreted from neuronal soma or axons in vivo, given that vesicular secretion of neurotransmitters are predominantly released from axon terminals. By employing sciatic nerve injection model to selectively label spinal motor neuron (SMN) exosomes and intravitreal injection to selectively label retina ganglion cells (RGC), Rashed will specifically investigate and compare whether neuronal exosomes are differentially released from either somatodendritic or axon terminal compartments and whether these exosomes are also differentially taken up into surrounding astrocytes. Understanding the predominant location of exosome secretion from such a polarized cell type as neurons will give important insights into the potential function of those exosomes. By carrying out this project, it will give him the opportunity to understand how to see a project through from conception to finish, learn various injection techniques, deal with the troubleshooting and optimization that are always needed in scientific experiments and learn the best ways to analyze images and data. Overall, this diversity supplement award will greatly prepare Rashed for his research career development.