Molecular Biology and Cytometry Research Shared Resource

分子生物学和细胞计量学研究共享资源 分子生物学和细胞计数研究共享资源（MBCR SR）是一个由机构管理的 中央核心设施，协助研究人员在研究的各个方面，包括初步项目咨询， 协助实验设计、方案和仪器使用培训、样品制备、数据 收集并协助分析和解释结果。在Ken Jones博士的指导下， MBCR SR为斯蒂芬森癌症中心（SCC）成员和其他 研究人员：1）DNA测序和基因组学核心提供高保真，下一代核酸 测序数据; 2）蛋白质组学核心为生物分子鉴定提供支持，包括复杂的 蛋白质组学分析，翻译后修饰鉴定，高分辨分子量 测定，大生物分子质量测量，LC-UV-MS分析（定性或定量）， 通过蛋白水解消化（凝胶内或溶液中）、纳米LC/MS/MS和数据库搜索进行蛋白质鉴定，以及 肽测序; 3）流式细胞术和成像核心提供流式细胞术和显微镜支持 用于蛋白质表达、细胞周期和细胞信号转导的亚细胞研究; 4）癌症功能基因组学 核心提供癌细胞的高通量活细胞功能表征，包括反向蛋白质组学 阵列建立、绝对基因定量和拷贝数测定、核酸和蛋白质 电泳和成像，平板上高通量测定的多模式筛选，高通量，高通量， 细胞中亚细胞定位蛋白的含量筛选、活细胞的代谢分析和 癌细胞的克隆形成测定集落; 5）生物信息学核心提供先进的计算 多组学数据分析（基因组学、蛋白质组学、代谢组学），以及6）质谱核心是一个 新生核心于2021年启动，作为专门从事代谢物和小分子分析的机构核心 使用最先进的质谱仪该SR已成功获得资金购买 用于从头发现和靶向分析的代谢组学质谱。MBCR SR位于斯坦顿 L.俄克拉荷马州大学健康科学中心（OUHSC）的青年生物医学研究中心（BRC） 校园这个中心位置为SCC的实验室提供了方便的MBCR SR访问 （布拉迪斯拉发区域中心37名成员，邻近的生物医学科学大楼15名成员）。期间 报告期内，78个正式和准SCC成员使用了该设施，其中69个（88%）具有同行资格， 审查赠款支助。由于这是一个机构管理的核心，支持整个OUHSC校园， 值得注意的是，SCC正式成员和准成员（n=78）占总数的三分之一以上（35%）， 该中心设施的用户（n=222）。SCC附属成员（非CCSG成员）占另一个 占总使用量的27（12%）。总的来说，这些关注癌症的成员占到目前为止最大的数量， OUHSC任何部门或中心的使用百分比。

MOLECULAR BIOLOGY AND CYTOMETRY RESEARCH SHARED RESOURCE: ABSTRACT The Molecular Biology and Cytometry Research Shared Resource (MBCR SR) is an institutionally managed central core facility that assists investigators in all aspects of research, including initial project consultation, assistance with experimental design, training in protocols and instrumentation usage, sample preparation, data collection and assistance with analysis and interpretation of results. Under the direction of Ken Jones, PhD, the MBCR SR provides six important core services to Stephenson Cancer Center (SCC) members and other investigators: 1) the DNA Sequencing and Genomics Core provides high-fidelity, next-generation nucleic acid sequencing data; 2) the Proteomics Core provides support for biomolecule identification, including complex proteomics analysis, post-translational modification identification, high-resolution molecular weight determination, large biomolecular mass measurements, LC-UV-MS analysis (qualitative or quantitative), protein identification by proteolytic digest (in-gel or in solution), nano LC/MS/MS and database search, and peptide sequencing; 3) the Flow Cytometry and Imaging Core provides flow cytometry and microscopy support for subcellular studies on protein expression, cell cycle and cell signaling; 4) the Cancer Functional Genomics Core provides high-throughput live cell functional characterization of cancer cells, including reverse proteomics array establishment, absolute gene quantification and copy number determination, nucleic acid and protein electrophoresis and imaging, multimodal screening for high-throughput assay on plates, high-throughput, high- content screening of subcellular localized proteins in cells, metabolic analysis of live cells and quantification of clonogenic assay colonies of cancer cells; 5) the Bioinformatics Core provides advanced computational analyses of multi-omic data (Genomic, Proteomic, Metabolomic), and 6) the Mass Spectrometry Core is a nascent core initiated in 2021 as an institutional core specializing in metabolite and small molecule analysis using state-of-the-art mass spectroscopy. This SR has been successful in obtaining funds to purchase metabolomic mass specs for de novo discovery and targeted analysis. The MBCR SR is located in the Stanton L. Young Biomedical Research Center (BRC) on the University of Oklahoma Health Sciences Center (OUHSC) campus. This central location provides convenient access to the MBCR SR for the SCC’s laboratory-based members (37 members in BRC, 15 members in the adjacent Biomedical Sciences Building). During the reporting period, 78 full and associate SCC members utilized this facility, with 69 (88%) of these having peer- reviewed grant support. Since this is an institutionally managed core that supports the entire OUHSC campus, it is important to note that full and associate SCC members (n=78) accounted for over a third (35%) of the total users of this centralized facility (n=222). SCC affiliate members (non-CCSG members) accounted for another 27 (12%) of total usage. Combined, these cancer focused members account for by far the largest number and percentage usage by any department or center at OUHSC.