Cornell ME/CFS Collaborative Research Center

项目概要：总体 尽管全世界数百万人遭受了巨大的痛苦，但肌痛的根本原因是 脑脊髓炎/慢性疲劳综合征（ME/CFS）是未知的，缺乏有效的治疗方法。 ME/CFS的特征是衰弱性疲劳、肌肉骨骼疼痛、头痛、认知困难 直立耐受不良和睡眠障碍由于缺乏简单的客观测试， 获得适当的诊断，并由于缺乏生物标志物来监测而抑制药物开发 实验疗法的有效性。为了获得ME/CFS的基本机制见解，我们将 利用来自四所不同学院的研究人员的经验，能力和不同背景， 康奈尔大学、佛罗里达大西洋大学、特殊外科医院和ME/CFS专家 医生。我们将利用已经从患者和对照组中获得的大量数据 在运动引起症状之前和之后，以及一组有价值的新样本。三 研究项目将寻求（1）使用尖端的多组学单细胞分析来检查细胞中的改变， ME/CFS肌肉中发生的类型、基因表达和细胞-细胞相互作用（项目1），（2）鉴定组织 通过表征释放到循环中的RNA，以及（3）鉴定运动后ME/CFS中的损伤 ME/CFS患者细胞外囊泡的RNA和蛋白质货物可能改变靶细胞的功能 （项目2）和（4）使用基因组和计算方法更好地了解基因调控 导致ME/CFS免疫失调的机制，并系统地鉴定ME/CFS特异性 改变整个免疫系统的信号（项目3）。这三个研究项目得到支持 由一个研究核心，将作为基因组学技术专业知识，试剂和服务的资源， 用于数据管理和综合分析。在所有研究中进行了多组学分析和预测建模 这三个项目将为未来治疗和诊断测试的发展提供基础。都是坚持 将通过一个行政核心协调各项活动，这将促进内部的协同作用和一体化。 该中心，同时也是与其他ME/CFS合作研究中心合作的平台， a患者/倡导者/护理人员委员会，其他ME/CFS研究人员和数据管理协调 中心行政核心还将负责外联活动， 在研究界，卫生专业人员和社区内对ME/CFS的认识和理解 公众，并将管理一个试点项目计划，旨在把新的想法和研究人员纳入 ME/CFS字段。

Project Summary: Overall Despite the enormous suffering endured by millions of people worldwide, the underlying causes of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) are unknown and effective therapies are lacking. ME/CFS is characterized by debilitating fatigue, musculoskeletal pain, headaches, cognitive difficulties orthostatic intolerance, and sleep disturbances. The absence of simple objective tests prevents many from obtaining an appropriate diagnosis and inhibits drug development because of the lack of biomarkers to monitor the efficacy of experimental therapies. In order to gain fundamental mechanistic insights into ME/CFS, we will leverage the experience, capabilities and varied backgrounds of researchers from four different colleges at Cornell University, Florida Atlantic University, the Hospital for Special Surgery, and an ME/CFS expert physician. We will take advantage of an enormous amount of data already obtained from patients and controls both before and after symptom provocation through exercise, as well as a valuable set of new samples. Three research projects will seek to (1) use cutting-edge multi-omic single cell profiling to examine alterations in cell types, gene expression, and cell-cell interactions that occur in ME/CFS muscle (Project 1), (2) identify tissue injured in ME/CFS following exercise through characterization of RNA released into circulation and (3) identify the RNA and protein cargo of extracellular vesicles in ME/CFS patients that may alter function of target cells (Project 2) and (4) Use genomic and computational methods to better understand the gene regulatory mechanisms that result in immune dysregulation in ME/CFS and systematically identify ME/CFS-specific alterations in signaling across the immune system (Project 3). These three research projects are supported by a Research Core that will act as a resource for genomics technology expertise, reagents, and services and for data management and integrated analysis. Multi-omic analysis and predictive modeling carried out in all three Projects will provide a foundation for future development of therapeutics and diagnostic tests. All Center activities will be coordinated through an Administrative Core, which will foster synergy and integration within the Center, while also being the platform for collaboration with other ME/CFS Collaborative Research Centers, a Patient/Advocate/Caregiver Committee, other ME/CFS researchers, and the Data Management Coordinating Center. The Administrative Core will also be responsible for outreach activities that are designed to increase awareness and understanding of ME/CFS within the research community, health professionals, and the general public, and will administer a pilot project program designed to bring new ideas and researchers into the ME/CFS field.