Core 1: Systems Pathology Core

核心1，系统病理学-摘要 癌症风险、发病率、治疗反应和结果的性别差异是众所周知的，但很少。 明白了。与膀胱癌有关的性别差异涉及性染色体、性别 激素和肿瘤微环境，包括免疫细胞和表观基因组的改变。这个 系统病理学核心(核心1)的首要目标是通过提供 简化和协调人和鼠生物检疫的收集、处理和分析，以实现 计划项目中概述的目标。该核心将由Anil Parwani博士(俄亥俄州立大学)共同领导 韦克斯纳医疗中心[OSUMC])和谢尔盖·格里文尼科夫博士(雪松-西奈医疗中心[CSMC])和威尔 为这一多机构项目提供协调服务。完成核心目标，避免重复 在服务方面，Core 1将与OSU的资源密切合作，包括比较病理学和数字 影像共享资源、病理科、免疫监测和发现平台，以及 合作人体组织网络中西部网络。在CSMC，核心1将利用流中的资源 细胞学、高级基因组学、质量细胞学资源共享及生物库翻译 研究核心。 系统病理学核心的具体目标和服务如下： 具体目标1.作为一个高质量的生物信息库，收集、处理、储存和分发注释 用于计划研究的人和小鼠膀胱癌标本及其衍生物。 具体目标2.为分析人和小鼠膀胱癌提供组织学和病理学服务 免疫组织化学和免疫荧光结合病理特征和免疫荧光检测 空间测绘(通过编制索引进行联合检测[法典])。这个核心还将为病理学家提供诊断 咨询、项目规划、方法开发、评分、口译和手稿写作。 具体目标3.提供流式细胞仪和单细胞分析服务，包括多参数流动 细胞分析和分类、单细胞RNA测序(ScRNAseq)、转录本细胞索引 和表位测序(CITEseq)，使用测序分析转座酶可访问的染色质 (ATACseq)、质量细胞术(CyTOF)和标准分子分析。核心将协调处理 以及跨计划站点的分析技术，以确保数据完整性和统一结果。 系统病理学核心将与行政核心和数据科学核心密切合作， 其将提供稳健的数据管理和对在此获取的数据的分析。

CORE 1, SYSTEMS PATHOLOGY – SUMMARY Sex differences in cancer risk, incidence, therapeutic response, and outcomes are well-known but poorly understood. Sex disparities relating to bladder cancers involves an interplay between sex chromosomes, sex hormones, and tumor micro-environment, including the immune cells and epigenomic alterations. The overarching goal of the Systems Pathology Core (Core 1) is to support Program investigators by providing streamlined and harmonized collection, processing and analysis of human and murine biospecimens to achieve the aims outlined in the Program projects. This core will be co-led by Dr. Anil Parwani (The Ohio State University Wexner Medical Center [OSUMC]) and Dr. Sergei Grivennikov (Cedars-Sinai Medical Center [CSMC]) and will provide coordinated services for this multi-institutional Program. To accomplish core goals and avoid duplication of services, Core 1 will work closely with resources at OSU, including the Comparative Pathology and Digital Imaging Shared Resource, the Pathology Department, the Immune Monitoring and Discovery Platform, and the Cooperative Human Tissue Network Midwestern network. At CSMC, Core 1 will utilize resources in the Flow Cytometry, Advanced Genomics, and Mass Cytometry Shared Resources as well as Biobank Translational Research Core. The specific aims and services for the Systems Pathology Core are: Specific Aim 1. Act as a high-quality biorepository to collect, process, bank, and distribute annotated human and murine bladder cancer specimens and their derivatives for Program research. Specific Aim 2. Provide histology and pathology services to analyze human and murine bladder cancer biospecimens using immunohistochemistry and immunofluorescence with pathological characterization and spatial mapping (via Co-Detection by Indexing [CODEX]). This core will also provide pathologist diagnostic consultation, project planning, method development, scoring, interpretation, and manuscript writing. Specific Aim 3. Provide flow cytometry and single cell analysis services including multiparameter flow cytometry analysis and sorting, single cell RNA sequencing (scRNAseq), Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITEseq), Assay for Transposase-Accessible Chromatin using sequencing (ATACseq), mass cytometry (CyTOF), and standard molecular analyses. The core will harmonize processing and analysis techniques across Program sites to ensure data integrity and unified results. The Systems Pathology Core will work closely with the Administrative Core and the Data Science Core, which will provide robust data management and analysis of the data acquired herein.