ShEEP Request for A Namocell PALA Single CellDispenser

我们需要Namocell PALA单电池分配器，这是一种台式仪器， 将创新的微流控技术与流式细胞仪和液体分配相结合。它可以 在几分钟内将单个电池分类并分配到96或384孔板中。它的低压运行(&lt；2 PSI)保持了细胞的活力和完整性。分离特定细胞群的能力是 对现代基础和应用生物医学研究具有无可估量的价值。微流控液滴分配器 在微环境中提供单细胞的小型化、高通量隔离， 可用于超灵敏和快速的分析。这些单元非常适合于各种类型的 单细胞应用包括具有改进的生长的细胞克隆，产生高产 单细胞基因组文库、特定目标细胞或稀有细胞群体的快速批量分选， 以及从最少100个细胞中回收单个细胞。 单电池分配器目前在几个学术和行业环境中使用。近期 联邦支持的设施对Namocell单电池分配器的收购包括 NIH/NIDA和弗吉尼亚州阿肯色州小石城。使用PALA单细胞分配器获得的数据将 既适用于细胞培养，也适用于人类疾病的啮齿动物模型研究。因此， 我们希望它将帮助我们破译这些疾病是如何发展或进展的，以及帮助 为患有这些疾病的退伍军人确定新的治疗机会。 JJP VAMC Namocell PALA单电池分配器的预期研究应用 范围从同质细胞群体的批量分选到单细胞分离。批量分拣是 对高度浓缩的特定细胞类型的初始审讯很有用。我们预计摩根大通VAMC 研究人员使用人类疾病的啮齿动物模型，包括神经、肾脏、 新陈代谢或与癌症相关的将使用PALA对特定或罕见细胞进行批量分选 群体，特别是那些居住在具有不同细胞类型的器官中的群体[主要用户：JG， CC、GP、RR]。批量分选还可用于识别在培养中操作的标记为 通过荧光活性，如表达特定荧光蛋白(FP)的转基因细胞。 PALA中的双激光系统将允许选择表达双外源基因的细胞 具有不同FP替代物的蛋白质，无需长时间的药物选择， 否则会引入混杂因素[主要用户：JG、CC、RR]。 Namocell PALA单细胞分配器也将用于单细胞的纯化。 单细胞分析可以区分不同细胞群体中的信号和噪声，并且 从而促进从人的组织或血液中获得稀有细胞群体的多组学研究 健康或疾病动物模型[主要使用：JG、CC、GP、RR]。PALA还将促进 在定义的微环境中实现单细胞的高吞吐量隔离，这是可以利用的 用于超灵敏和快速检测[主要用户：JG、GP、RR]。最后，我们期待着伟大的 利用PALA克隆精密基因工程转化细胞的兴趣 或诱导多能干细胞(IPSC)，这可能为结构功能研究提供见解 以及人类基因中发现的非同义变异对人类疾病的贡献 研究，分别[主要用户：JG、CC、GP、RR]。

We are requesting a Namocell PALA Single Cell Dispenser, a benchtop instrument that combines innovative microfluidics technology with flow cytometry and liquid dispensing. It can sort and dispense single cells into 96 or 384-well plates in minutes. Its low-pressure operation (<2 psi) preserves cell viability as well as integrity. The ability to isolate specific cell populations is invaluable for modern basic and applied biomedical research. Microfluidic droplet dispensers provide for miniaturized, high-throughput isolation of single cells in a microenvironment, which can be leveraged for ultra-sensitive and rapid assays. These units are ideal for a wide range of single cell applications including cell cloning with improved outgrowth, generation of high-yield single cell genomics libraries, rapid bulk sorting of specific target cells or rare cell populations, and recovery of single cells from as few as 100 cells. Single cell dispensers are currently in use in several academic and industry settings. Recent acquisition of Namocell Single Cell Dispensers by federally supported facilities include NIH/NIDA and Arkansas Little Rock VA. Data obtained with the PALA Single Cell Dispenser will have applications for both cell culture as well as rodent model studies of human disease. As such, we expect it will aid us in deciphering how these diseases develop or progress as well as help in identifying new treatment opportunities for Veterans with these diseases. The expected research applications for the Namocell PALA Single Cell Dispenser at JJP VAMC will range from bulk sorting of homogenous cell populations to single cell isolation. Bulk sorting is useful for initial interrogations of highly enriched, specific cell types. We expect JJP VAMC investigators who use rodent models of human diseases including those with neurological, renal, metabolic, or cancer relevance will use the PALA for bulk sorting of specific or rare cell populations, particularly those residing in organs with heterogenous cell types [Major Users: JG, CC, GP, RR]. Bulk sorting is also useful for identifying cells manipulated in culture that are marked by fluorescent activity, such as transfected cells expressing specific fluorescent proteins (FP). The dual laser system in the PALA will allow for selection of cells expressing dual exogenous proteins with distinct FP surrogates without the need for prolonged drug selection, which otherwise can introduce confounding factors [Major Users: JG, CC, RR]. The Namocell PALA Single Cell Dispenser will also be used for purification of single cells. Single-cell analysis can discriminate signal from noise in a heterogeneous cell population and thereby facilitate multiomic studies of rare cell populations obtained from tissues or blood of healthy or disease animal models [Major Users: JG, CC, GP, RR]. The PALA will also facilitate high-throughput isolation of single cells in a defined microenvironment, which can be leveraged for ultra-sensitive and rapid assays [Major Users: JG, GP, RR]. Finally, we anticipate great interest in the use of the PALA for cloning of precision genetically engineered transformed cells or induced pluripotent stem cells (iPSC), which may offer insights into structure-function studies as well as human disease contributions from nonsynonymous variants identified in human genetic studies, respectively [Major Users: JG, CC, GP, RR].