Actin filament mechanics and branched network turnover

总结 肌动蛋白丝组装成Arp 2/3复合分支丝网络， 基本的真核细胞过程，包括运动、胞吞和胞质分裂。成员 肌动蛋白解聚因子同源性（ADF-H）结构域蛋白家族，包括cofilin和glial 成熟因子（GMF），加速分支肌动蛋白丝网络重组和周转。Cofilin和 GMF通过脱支阿普2/3复合物加速网络重构。Cofilin通过以下方式加速网络周转： 分裂丝并增加丝末端的浓度，其中亚基添加和解离。 该MIRA提案整合了生物化学和生物物理方法，包括电子冷冻显微镜， 微流控技术，以及最近开发的显微磁柱分析，以开发预测模型， 肌动蛋白丝和丝网络力学，重塑和断裂。关于《公约》的一般原则 肌动蛋白丝弹性、构象和调节蛋白占有率之间的关系将从 这项工作

SUMMARY The assembly of actin filaments into Arp2/3 complex-branched filament networks powers numerous fundamental eukaryotic cellular processes, including motility, endocytosis, and cytokinesis. Members of the actin depolymerization factor homology (ADF-H)-domain protein family, which includes cofilin and glial maturation factor (GMF), accelerate branched actin filament network reorganization and turnover. Cofilin and GMF accelerate network remodeling by debranching Arp 2/3 complex. Cofilin accelerates network turnover by fragmenting filaments and increasing the concentration of filament ends where subunits add and dissociate. This MIRA proposal integrates biochemical and biophysical approaches, including electron cryo-microscopy, microfluidics, and a recently developed microscopic magnetic cylinders assay, to develop predictive models of actin filament and filament network mechanics, remodeling and fragmentation. General principles regarding the relationship between actin filament elasticity, conformation and regulatory protein occupancy will emerge from this work.