EARLY CELLULAR AND MOLECULAR EVENTS IN UVEITIS

描述(摘自申请者的摘要)：葡萄膜炎是主要的 在美国造成法律盲目的原因。对首字母的说明 葡萄膜炎发展过程中的事件可能提供新的治疗方法 这种致盲疾病的管理方法。这些研究 是基于一种假设，即骨髓来源的细胞启动 通过向CD4+T细胞和这样的骨骼递送视网膜抗原而引起的葡萄膜炎 骨髓来源的APC可能存在于视网膜和/或脉络膜中。这 假说将通过收养的骨髓嵌合体进行验证 S抗原特异性T细胞株的转移。具体地说， 应用将：(1)确定骨髓来源细胞的作用 在实验性自身免疫性葡萄膜视网膜炎(EAU)中 T细胞的嵌合体，(2)鉴定和定位骨髓来源的 视网膜和脉络膜中的细胞和(3)决定细胞因子的分布 这些细胞在葡萄膜炎的发展过程中。这将是 由一组具有以下专业知识的调查人员完成 免疫病理学、免疫组织化学、原位分子技术和 分子生物学。

DESCRIPTION (Adapted from applicant's abstract): Uveitis is the leading cause of legal blindness in the United States. Elucidation of initial events in the development of uveitis could provide new therapeutic approaches to the management of this blinding disease. These studies are based on the hypothesis that bone marrow-derived cells initiate uveitis by presenting retinal antigens to CD4+T-cells and such bone marrow-derived APCs may be present in the retina and/or choroid. This hypothesis will be tested using bone marrow chimeras with adoptive transfer of S-antigen specific T-cell lines. Specifically, the application will: (1) determine the role of bone marrow-derived cells in experimental autoimmune uveoretinitis (EAU) by transfer of specific T-cells to chimeras, (2) identify and localize the bone marrow-derived cells in retina and choroid and (3) determine the cytokine profile of these cells during the development of uveitis. This will be accomplished by a team of investigators with expertise in immunopathology, immunohistochemistry, in situ molecular techniques and molecular biology.