ARROW, A NEW WINGLESS SIGNALING COMPONENT

这项工作的长期目标是阐明分子 果蝇发育过程中花纹形成的基础。 无翼信号的一个遗传成分的特征 果蝇胚胎表皮中的途径将用于定义 管理发展决策所需的要素 无脊椎动物，以此类推，脊椎动物。金黄色葡萄球菌的分子克隆 Arrow，一个新发现的无翼途径的成员，将是 并将促进后续研究，旨在进一步 确定箭头在无翼路径中所起的作用。新一代人 针对Arrow蛋白的抗体将有助于确定其 细胞定位。遗传上位性与胚胎显微注射 将提供有关该位置的信息，可能还会提供 箭在无翼路径中的作用机制。其中的影响 这项工作的关键在于这种遗传级联可能会 提供对脊椎动物发育机制的见解 无翼途径利用一种新的信号转导形式， 在进化上高度保守。

The long-term objectives of this work are to elucidate the molecular basis of pattern formation during development in Drosophila melanogaster. The characterization of a genetic component of the Wingless signaling pathway in the Drosophila embryonic epidermis will serve to define elements required for regulation of developmental decisions in invertebrates and, by analogy, vertebrates. The molecular cloning of arrow, a newly identified member of the Wingless pathway, will be performed and will facilitate subsequent studies designed to further identify the role arrow plays in the Wingless pathway. The generation of antibodies specific to the Arrow protein will aid in determining its cellular localization. Genetic epistasis and embryonic microinjection will provide information regarding the location, and possibly the mechanism of action, of arrow in the Wingless pathway. The implications of this work lie in the possibility that this genetic cascade will provide insights into mechanisms of vertebrate development as the Wingless pathway utilizes a novel form of signal transduction and is highly conserved evolutionarily.