Studies Of DNA Mismatch Repair

这一年，我们在DNA错配修复和核糖核苷酸切除修复六个方面的研究取得了实质性进展。(1)研究了酵母Pms1蛋白的核酸内切酶活性在MMR过程中产生链识别信号中的作用。(2)我们研究了MMR和RER中Rad27皮瓣内切酶活性的突变特异性。(3)为了研究冈崎片段成熟的一个方面，我们研究了Msh2在含有DNA连接酶1突变的酵母菌核基因组单碱基突变的MMR中的作用。(4)我们已经调查了全基因组测序数据库，以寻找证据，证明一些点突变是由于单核苷酸碱基的短暂错配引起的。(5)利用全基因组测序，我们已经开始研究DNA外切酶1在复制错误的MMR中的作用，假设这种5-外切酶优先修复DNA聚合酶α造成的错误。(6)我们在RER上发表了一篇评论文章。

This year, we made substantial progress on investigations of six aspects of DNA Mismatch Repair and Ribonucleotide Excision Repair. (1) We investigated the role of the endonuclease activity of the yeast Pms1 protein in generating the signal for strand discrimination during MMR. (2) We investigated the mutational specificity of the Rad27 flap endonuclease activity in both MMR and RER. (3) To investigate one aspect of Okazaki fragment maturation, we investigated the role of Msh2 in MMR of single base mutations across by nuclear genome of yeast strains containing a mutation in DNA ligase 1. (4) We have investigated whole genome sequencing databases for evidence that some point mutations arise as a consequence of transient misalignments in mononucleotide runs of bases. (5) Using whole genome sequencing, we have begun to investigate the role of DNA exonuclease 1 in MMR of replication errors, with the hypothesis that this 5-exonuclease preferentially repairs errors made by DNA polymerases alpha. (6) We published a review article on RER.