Core 3: Chemical Biology & Materials Tools (CBMT)

项目摘要 用于探测、操纵和成像生物系统的化学生物学工具可以提供 对基本过程的深刻洞察。化学二聚化与蛋白质定位 用于分析物检测的荧光探针和传感器，以及用于 扰乱生物途径和过程已经成为收集的重要研究工具 更深入地了解生物过程。这些光学触发变体的发展 重要的化学生物学工具提供了新的和更有力的探测方法， 以新的空间和时间水平操纵和成像关键途径和过程 控制以前不可用。尽管分子化学生物学方法的效用， 由于需要按需合成， 和分子定制取决于正在研究的途径和过程， 缺乏商业可用性。拟议的化学生物学和材料核心将解决 这些问题通过直接与个别项目的方案进行设计， 综合并实施为项目定制的尖端化学生物学工具 研究目的。化学生物学核心也将作为整个奖项的资源 开发新方法和实施与MAC核心协同的关键技术的时期 和个别项目。在目标1中，核心将开发用于光遗传控制的化学二聚体 关键的生物分子定位和激活事件。在目标2中，核心将开发基于肽的 用于询问关键生物途径和过程的化学探针。

Project Summary Chemical Biology tools for probing, manipulating, and imaging biological systems can provide deep insights into fundamental processes. Chemical dimerization and protein localization strategies, fluorescent probes and sensors for analyte detection, and small molecules for perturbing biological pathways and process have become important research tools for gleaning deeper insight into biological processes. The development of optically triggered variants of these important chemical biology tools has provided new and more powerful approaches to probing, manipulating, and imaging key pathways and processes with new levels of spatial and temporal control not previously available. Despite the utility of molecular chemical biology approaches, many of the tools and reagents are not readily available due to the need for on-demand synthesis and molecular customization depending on the pathways and processes being studied and the lack of commercially availability. The proposed Chemical Biology and Materials Core will solve these problems by working directly with the individual projects of the program to design, synthesize, and implement cutting edge Chemical biology tools customized to the project research aims. The Chemical Biology Core will also serve as a resource throughout the award period to develop new approaches and implement key technologies in synergy with the MAC core and individual projects. In Aim 1 the core will develop chemical dimerizers for optogenetic control of key biomolecule localization and activation events. In Aim 2 the core will develop peptide based chemical probes for interrogation of key biological pathways and processes.