Bioconjugation and self-assembly of carbon nanotubes

碳纳米管的生物共轭和自组装

• 批准号: 7678162
• 负责人: David Michael Chenoweth
• 金额: $ 4.52万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7678162
• 关键词: Area; Biocompatible Materials; Biosensor; Carbohydrates; Carbon; Carbon Nanotubes; Chemicals; Development; Drug Delivery Systems; Genes; Goals; Gold; Health; Human; Hybrids; Knowledge; Lead; Mediating; Medicine; Methods; Modification; Nucleic Acids; Oligonucleotides; Property; Proteins; Reagent; Research; Small Interfering RNA; System; Techniques; Transfection; Transistors; Viral; base; cell growth; method development; molecular recognition; nanoparticle; novel; practical application; self assembly; sensor; small molecule; tissue regeneration

DESCRIPTION (provided by applicant): This proposal describes methods for the efficient and controlled bioconjugation and self-assembly of carbon nanotubes (CNTs). The proposed research will focus on the development of mild chemical methods for the covalent attachment of biomolecules (nucleic acids, proteins, carbohydrates, etc.) and small molecules with solubilizing properties to carbon nanotubes and their self-assembly into new hybrid materials. These hybrid DNA-CNT materials will be characterized and their molecular recognition properties explored for use as electrochemical or field-effect-transistor based biosensors. The first aim of the proposal is the development of methods for the functionalization of carbon nanotubes with biomolecules (DMA). The second aim of the proposal is the development of new DMA-based carbon nanotube assembly techniques. The last aim is to study the enzymatic modification and molecular recognition properties of these new materials with proteins and small molecules, paving the way toward their application as highly sensitive biosensor systems. The three primary architectural strategies for assembling DMA materials are linear, branching, and multibranched higher order motifs. The linear strategy is the simplest conceptually, and has been applied recently to the self-assembly of gold nanoparticles into highly ordered crystalline arrays. While this strategy has been demonstrated for gold nanoparticles, the self assembly of DNA-carbon nanotube materials, with high degrees of order, has yet to be achieved and represents a much needed area of research. The successful self-assembly of DNA-carbon nanotube conjugates into highly ordered materials would constitute a major advance in the field and is the principle goal of the proposed research. The discovery of novel biomaterials and detailed knowledge of their fundamental properties could lead to practical applications in human health and medicine. DNA-CNT hybrid materials have many potential applications some of which include substrates for cell growth or tissue regeneration, drug delivery systems, gene transfection reagents, nanotube (non-viral) mediated oligonucleotide transport for siRNA, and electrochemical or field-effect-transistor based biosensors.

描述（由申请人提供）：该提案描述了碳纳米管（CNT）的有效且受控的生物共轭和自组装的方法。拟议的研究将重点开发温和的化学方法，用于将生物分子（核酸、蛋白质、碳水化合物等）和具有增溶特性的小分子共价连接到碳纳米管上，并将其自组装成新型混合材料。这些 DNA-CNT 混合材料将被表征，并探索它们的分子识别特性，以用作基于电化学或场效应晶体管的生物传感器。该提案的首要目标是开发生物分子（DMA）功能化碳纳米管的方法。该提案的第二个目标是开发新的基于 DMA 的碳纳米管组装技术。最后一个目标是研究这些新材料与蛋白质和小分子的酶促修饰和分子识别特性，为其作为高灵敏度生物传感器系统的应用铺平道路。组装 DMA 材料的三种主要结构策略是线性、分支和多分支高阶基序。线性策略在概念上是最简单的，最近已应用于金纳米粒子自组装成高度有序的晶体阵列。虽然这种策略已在金纳米粒子上得到证实，但高度有序的 DNA-碳纳米管材料的自组装尚未实现，这代表了一个急需的研究领域。 DNA-碳纳米管缀合物成功自组装成高度有序的材料将构成该领域的重大进步，也是本研究的主要目标。新型生物材料的发现及其基本特性的详细了解可能会导致其在人类健康和医学领域的实际应用。 DNA-CNT 杂化材料具有许多潜在的应用，其中一些包括细胞生长或组织再生的基质、药物输送系统、基因转染试剂、纳米管（非病毒）介导的 siRNA 寡核苷酸运输，以及基于电化学或场效应晶体管的生物传感器。