Cardiovascular Immunology Research Core (Core B)

心力衰竭进展是一个复杂的生物过程，由适应性不良的心肌细胞引起。 对损伤的反应，再加上成人心脏不能替换丢失或受损的心肌细胞。 可以想象，确定控制这两个看似不相关的过程的共同途径将 对预防甚至逆转心力衰竭进展的治疗策略产生深远影响。数不胜数 提议的财团成员和其他人的观察支持这样一种观点，即内生的 新生哺乳动物心脏的增殖能力在出生后早期就会减弱，从 心肌细胞发生从肥大到肥大的生长。拟议财团的成员和 其他人之前也证明了与激活先天免疫反应有关的机制可能 在心肌细胞肥大、死亡甚至刺激新生心肌细胞中起作用 一代。然而，参与心肌细胞周期停滞的机制是否也在 不适应的心肌细胞对损伤的反应尚不清楚。事实上，炎症性疾病的关键成分 反应似乎也是损伤后心脏恢复活力的基础，对愈合也有积极作用。 刺激心肌细胞增殖。因此，目前的提案将5个小组结合在一起 在心肌重塑、再生和免疫学方面的专业知识，总体目标是确定其作用 免疫反应信号在心脏生长和再生调节中的作用。4个项目负责人是 心血管生物学家，因此在网络中拥有强大的免疫学存在对其 成功，不仅要提供技术专长和试剂，还要起到至关重要的咨询作用。堆芯 B将由著名免疫学家James Chen博士领导，他将发挥关键作用，为 在与免疫学研究有关的所有事项上向项目负责人提供后勤支持。这是一个关键的和 当前计划项目的创新设计，因为它将允许4个项目负责人无缝整合 将复杂的免疫学分析和概念纳入他们的研究计划。因此，核心B将提供 一些基本的支持服务，包括免疫细胞的分离和鉴定，执行基因 表达分析研究，为免疫学分析提供关键试剂，并为 学习设计和解释。免疫学目标将在网络中发挥宝贵的作用， 增强协同效应，最大限度地提高所有网络项目的工作效率

Heart failure progression is a complex biological process that is precipitated by the maladaptive myocardial response to injury, compounded by failure of the adult heart to replace lost or damaged cardiomyocytes. Conceivably, identifying common pathways that regulate these two seemingly unrelated processes would profoundly impact therapeutic strategies to prevent, and even reverse heart failure progression. Numerous observations by members of the proposed consortium and others support the notion that the endogenous capacity of the neonatal mammalian heart to proliferate fades in the early postnatal life as a switch from hyperplastic to hypertrophic growth of cardiomyocytes takes place. Members of the proposed consortium and others have also previously demonstrated that mechanisms linked to activation of innate immune response may play a role in cardiomyocyte hypertrophy, death and even stimulation of new cardiomyocyte generation. However, whether mechanisms involved in cardiomyocyte cell cycle arrest also play a role in the maladaptive cardiomyocyte response to injury is not known. Indeed, critical components of the inflammatory response appear to underlie both cardiac rejuvenation after injury through positive effects on healing, as well as stimulating cardiomyocytes to proliferate. Therefore, the current proposal brings together 5 groups with expertise in myocardial remodeling, regeneration and immunology with the overall goal of determining the role of immune response signaling in regulation of cardiac growth and regeneration. The 4 Project Leaders are cardiovascular biologist, and thus having a strong immunology presence in the network is paramount for its success, not only to provide technical expertise and reagents, but also to play a crucial consultancy role. Core B will be led by Dr. James Chen, a renowned immunologist, who will play the critical role of providing technical and logistic support to Project Leaders in all matters pertaining to immunological studies. This is a critical and innovative design of the current Program Project as it will allow the 4 Project leaders to seamlessly integrate complex immunological assays and concepts into their research program. As such, Core B will provide a number of essential support services including isolation and characterization of immune cells, performing gene expression analysis studies, providing critical reagents for immunological assays and providing consultation on study design and interpretation. The immunology goal will serve an invaluable role within the network that will enhance synergy, and maximize productivity of all Network Projects