Gnotobiotics, Microbiology and Metagenomics Core

项目摘要： Gnotobiotics，微生物学和宏基因组学核心（核心D），支持研究人员研究 微生物群在健康和疾病中的功能作用。核心D已经支持了超过100个感兴趣的团体， 评价宿主的微生物群影响宿主生理的作用机制， 病理生理过程 我们的资源包括微生物培养，表型分析，原发性 用于微生物代谢产物谱的样品，并为功能研究开发定义的财团，临床实验室 （CLIA）支持以微生物组为重点的临床试验的单位，基于序列分析的分子单位， 用于动物模型体内研究的无菌（无菌）小鼠设施，一个代谢单位， 小鼠代谢笼，身体组成和代谢研究，以及计算单元， 进行新的算法开发，以评估微生物群的纵向动态， 用于宿主微生物群研究的实验设计。 核心D为大波士顿地区的多个学术中心提供支持。

Project Summary: The Gnotobiotics, Microbiology and Metagenomics Core (Core D), supports investigators studying functional roles of the microbiota in health and disease. Core D has supported >100 groups interested in evaluating mechanisms of action by which the host’s microbiota affects host physiologic and pathophysiologic processes. Our resources include a Microbiology Unit for microbiologic culturing, phenotyping, analysis of primary samples for microbial metabolite profiles, and develop defined consortia for functional studies, a Clinical Lab (CLIA) Unit to support microbiome-focused clinical trials, a Molecular Unit for sequence-based analyses, a Gnotobiotic (germfree) mouse facility for in vivo studies in animal models, a Metabolic Unit to support mouse metabolic cage, body composition and metabolism studies, and a Computational Unit which undertakes novel algorithmic development to evaluate longitudinal dynamics of the microbiota and assist users in experimental design for host-microbiota studies. Core D provides support to multiple academic centers in the greater Boston area.