Translational Science Biocore

项目摘要/摘要 翻译科学生物核心(TSB)的使命是通过提供 全面的服务，能够对动物模型和人类进行细胞和分子表征- 衍生的生物制品。目标1是收集、注释和分发人类来源的生物标本。TSB运营 经机构审查委员会(IRB)批准，收集、存储、注释和分发各种人力资源 符合特定质量参数的组织和体液。在目前的癌症中心支持赠款中 (CCSG)周期，我们扩展了我们的方案，以增强患者的同意并改善获取组织的途径，并将 跨多个资源和存储库的临床数据。TSB被授权作为“诚实的经纪人” 为调查人员提供编码以保护患者机密性的临床注释。TSB还提供了 这些服务通过项目特定的、IRB批准的协议向UWCCC成员提供，例如由调查员发起 以及国家临床试验。目标2是构建和分布疾病特异性组织微阵列(TMA)。 TSB病理学家检查档案组织块以确定适合包含在每个TMA中的标本 然后由TSB工作人员建造。在当前的CCSG周期中，57个带有注释的临床数据的新TMA 创建，使总数达到115，这得益于购买了一台由 NIH S10奖(S10OD023526)。目标3是提供高质量的服务、专业知识和基础设施 细胞病理学、分子病理学、定量病理学和计算病理学。TSB病理学家和专家工作人员指导项目 开发、进行实验并解释利用人类和/或动物模型得出的数据 生物群落。我们广泛的专业知识和服务涵盖组织病理学、形态计量分析、定量 特异性分子表型、原位杂交、免疫组织化学和细胞系分析 身份验证。TSB包含每个服务所需的最先进的工具。在此期间 CCSG周期，TSB升级了其数字病理全切片扫描仪，并购买了系统以扩展空间 和分子图谱服务。对UWCCC的影响：在本CCSG周期内，TSB支持 对所有六个科学项目的129个独特的UWCCC计划成员进行的研究，分布在4800多个项目中 生物群落。这些成员拥有431项与癌症相关的研究资助，每年的直接成本总计6560万美元， 在这段时间内，包括142笔NCI补助金。通过将人类和动物病理核心结合在一起， 通过改进效率/协调，CCSG循环进入中央管理，我们增加了 提供服务的成员、收集的生物检疫剂的类型、处理/分发的样本数量以及 支持的方案/临床试验。我们增加了TMA的创建，并提供了新的空间和分子 简介服务，使成员能够进行创新的研究，推动UWCCC的使命。 CCSG的持续支持使我们能够为成员提供优先访问生物检疫的机会，范围广泛 低成本的病理学服务和专家咨询支持，以满足他们的研究需求。

PROJECT SUMMARY / ABSTRACT The mission of the Translational Science Biocore (TSB) is to facilitate cancer research by providing comprehensive services that enable cellular and molecular characterization of animal models and human- derived biospecimens. Aim 1 is to collect, annotate and distribute human-derived biospecimens. TSB operates with Institutional Review Board (IRB) approval to collect, store, annotate and distribute a wide variety of human tissues and body fluids that meet specified quality parameters. In the current Cancer Center Support Grant (CCSG) cycle, we expanded our protocol to enhance patient consenting and improve access to tissue and linked clinical data across multiple resources and repositories. TSB is authorized to serve as an ‘honest broker’ to provide investigators with clinical annotations that are coded to protect patient confidentiality. TSB also provides these services to UWCCC members with project-specific, IRB-approved protocols, such as investigator-initiated as well as national clinical trials. Aim 2 is to construct and distribute disease-specific tissue microarrays (TMAs). TSB pathologists review archival tissue blocks to identify specimens well suited for inclusion in each TMA which is then constructed by TSB staff. In the current CCSG cycle, 57 new TMAs with annotated clinical data were created, bringing the total to 115 which was facilitated by the purchase of an automated microarrayer funded by an NIH S10 award (S10OD023526). Aim 3 is to provide high quality services, expertise, and infrastructure in cellular, molecular, quantitative, and computational pathology. TSB pathologists and expert staff guide project development, conduct experiments, and interpret data that utilize human- and/or animal model-derived biospecimens. Our broad expertise and services span histopathology, morphometric analysis, quantitative analyses of specific molecular phenotypes, in situ hybridization, immunohistochemistry, and cell line authentication. TSB houses state-of-the-art instrumentation required for each of these services. During this CCSG cycle, TSB upgraded its digital pathology whole slide scanner and purchased systems to expand spatial and molecular profiling services. Impact on UWCCC: During the current CCSG cycle, TSB has supported the research of 129 unique UWCCC program members across all six scientific programs and distributed over 4,800 biospecimens. These members held 431 cancer-relevant research grants totaling $65.6M in annual direct costs, including 142 NCI grants, during this interval. By combining human and animal pathology cores from the previous CCSG cycle into central management with improved efficiency/coordination, we have increased the number of members served, types of biospecimens collected, number of samples processed/distributed, and the number of protocols/clinical trials supported. We have increased TMA creation and offer new spatial and molecular profiling services enabling members to perform innovative research that advances the UWCCC mission. Continued CCSG support allows us to provide members with priority access to biospecimens, extensive pathology services at low-cost and expert consultative support for their research needs.