CORE--COMPUTER MICROSCOPE

CORE-B拥有自动扫描、识别和测量物体的资源 直接从显微镜载玻片上以高分辨率拍摄。 它是一个独特 具有Ludl电动载物台的Zeiss轴平面显微镜的配置， 精细聚焦和滤光片更换; CCD摄像机; Perceptics图像采集和 处理系统;本地VAX集群;和大容量存储设备。 的 资源通过以太网连接到其他资源。 本地开发的软件 系统地扫描单个显微镜切片， 聚焦;处理图像的特征，如染色的细胞体、银 颗粒和神经过程;从扫描区域生成蒙太奇 不同的对象;数据的统计分析和表示; 数据到LONI，以便从串行部分进行对齐。 CORE-B将与 到CORE-C接收和处理来自共焦显微镜的图像， 到CORE-E发送和接收来自神经成像实验室的数据。 项目2、3和6将使用该资源来枚举粒子，如 银颗粒和HRP颗粒。 项目4、5和7将使用 计数细胞、纤维和银颗粒的资源。 项目1将 使用资源收集轴突的图像进行描述。

CORE-B has resources to automatically scan, recognize and measure objects at high resolution directly from microscopic slides. It is a unique configuration of a Zeiss axioplan microscope with Ludl motorized stage, fine focus and filter changer; CCD camera; Perceptics Image Capture and Processing System; Local VAX cluster; and mass storage devices. The resource is connected to others via ethernet. Locally developed software systematically scans individual microscopic sections with automatic focusing; process images for features such as stained cell bodies, silver grains, and neuronal processes; generates montages from scanned areas of different objects; statistical analyses and representations of the data; data to LONI for alignment from serial sections. CORE-B will be linked to CORE-C to receive and process images from the confocal microscope and to CORE-E to send and receive data from the Laboratory of NeuroImaging. Projects 2, 3 and 6 will use the resource to enumerate particles like silver grains and HRP granules. Projects 4, 5 and 7 will use the resource to enumerate cells, fibers and silver grains. Project 1 will use the resource to collect images of axons for depiction.