FUNCTIONAL ANATOMY OF LOCAL CIRCUITS IN BARREL CORTEX
桶状皮层局部电路的功能解剖
基本信息
- 批准号:3418745
- 负责人:
- 金额:$ 1.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-08-31 至 1993-09-30
- 项目状态:已结题
- 来源:
- 关键词:biomarker brain metabolism central neural pathway /tract computer program /software deoxyglucose digital imaging glutamate dehydrogenase hamsters image processing immunocytochemistry laboratory mouse neural information processing neural inhibition neural plasticity neuroanatomy sensory deprivation somesthetic sensory cortex stainings tissue /cell culture trigeminal nerve
项目摘要
The study of local circuits in cerebral cortex has relied on
physiological measures of the responses of single neurons to stimulation.
The obvious missing ingredient in current approaches to cortical function
is the ability to study the normal function of large groups of neurons
working together. We have developed an experimental procedure which
makes it possible, for the first time, to study the metabolic activity
of every immunohistochemically labeled neuron in a neural system of
interest. The procedure combines a high-resolution 2-deoxyglucose (2DG)
technique, immunostaining for glutamate decarboxylase (GAD) and other
antigens, and extensively automated data collection and analysis. These
materials allow a direct assessment of the extent of excitation and
inhibition in individual neurons. We propose to make extensive use of
this double-labeling technology to study local circuitry in the whisker-
related barrel field of rodent somatosensory cortex.
We will quantitatively map and analyze the cellular patterns of cortical
2DG labeling seen in behaving hamsters and mice. We will compare
cortices from normal, acutely whisker-trimmed, artificially stimulated,
and chronically whisker-trimmed rodents with respect to the underlying
distribution of GAD and other immunostains. We will generate complete
high resolution functional maps of the stimulated and surrounding
portions of the barrel field, enabling accurate comparison of the
metabolic activities in antigenically defined compartments across the
cortex. From the database represented by these maps, we will test a
number of specific hypotheses about cortical function and information
flow in the trigeminal system of the behaving animal.
A detailed comparison of our previous 2DG data and predictions derived
from single-unit physiology shows good agreement despite vast differences
in the two methods. We will pursue this line of evidence by looking for
parallels in 2DG/immunostained materials to functional groups of neurons
that have been physiologically characterized by other workers. We will
then examine by anatomical means the projection fields of excitatory and
inhibitory populations of neurons, and correlate the results of these
measures with the extent of excitation and inhibition in individual
neurons with different combinations of stimulated whiskers. We will
assess the specific contribution of cortical and subcortical circuitry
by comparing the activation patterns in various stations of the
trigeminal pathway with the patterns of whiskers remaining on the face.
Finally, we will characterize the plasticity in barrel cortex by
comparing the normal patterns of cortical labeling with those observed
following prolonged deprivation (whisker trimming) or artificial whisker
stimulation.
对大脑皮层局部回路的研究依赖于
单个神经元对刺激反应的生理测量。
当前皮质功能方法中明显缺失的成分
是研究大群神经元正常功能的能力
一起工作。 我们开发了一个实验程序
首次使研究代谢活动成为可能
神经系统中每个免疫组织化学标记的神经元
兴趣。 该程序结合了高分辨率 2-脱氧葡萄糖 (2DG)
技术、谷氨酸脱羧酶(GAD)免疫染色等
抗原,以及广泛自动化的数据收集和分析。 这些
材料允许直接评估激发程度和
对单个神经元的抑制。 我们建议广泛利用
这种双标记技术可以研究晶须中的局部电路
啮齿动物体感皮层的相关桶状场。
我们将定量绘制和分析皮质的细胞模式
在行为仓鼠和小鼠中观察到 2DG 标记。 我们会比较
正常的皮质,胡须急剧修剪,人工刺激,
以及长期修剪胡须的啮齿类动物
GAD 和其他免疫染色的分布。 我们将生成完整的
受刺激和周围环境的高分辨率功能图
桶场的部分,能够准确比较
跨抗原定义的区室的代谢活动
皮质。 从这些地图代表的数据库中,我们将测试
关于皮质功能和信息的具体假设的数量
行为动物三叉神经系统中的流动。
我们之前的 2DG 数据和预测的详细比较
尽管存在巨大差异,但单单位生理学显示出良好的一致性
两种方法中。 我们将通过寻找来追踪这一证据
2DG/免疫染色材料与神经元功能组的相似性
其他工人的生理特征。 我们将
然后通过解剖学手段检查兴奋性和
神经元的抑制群体,并将这些结果关联起来
衡量个体的兴奋和抑制程度
具有不同刺激胡须组合的神经元。 我们将
评估皮质和皮质下回路的具体贡献
通过比较各个站点的激活模式
三叉神经通路,脸上残留着胡须的图案。
最后,我们将通过以下方式来表征桶状皮层的可塑性:
将皮质标记的正常模式与观察到的进行比较
长期剥夺(胡须修剪)或人工胡须后
刺激。
项目成果
期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
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JAMES STACY MCCASLAND其他文献
JAMES STACY MCCASLAND的其他文献
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{{ truncateString('JAMES STACY MCCASLAND', 18)}}的其他基金
Development of Cortical Barrels in GAP-43 Deficient Mice
GAP-43 缺陷小鼠皮质桶的发育
- 批准号:
6529643 - 财政年份:2001
- 资助金额:
$ 1.97万 - 项目类别:
Development of Cortical Barrels in GAP-43 Deficient Mice
GAP-43 缺陷小鼠皮质桶的发育
- 批准号:
6774748 - 财政年份:2001
- 资助金额:
$ 1.97万 - 项目类别:
Development of Cortical Barrels in GAP-43 Deficient Mice
GAP-43 缺陷小鼠皮质桶的发育
- 批准号:
6395414 - 财政年份:2001
- 资助金额:
$ 1.97万 - 项目类别:
Development of Cortical Barrels in GAP-43 Deficient Mice
GAP-43 缺陷小鼠皮质桶的发育
- 批准号:
6637365 - 财政年份:2001
- 资助金额:
$ 1.97万 - 项目类别:
FUNCTIONAL ANATOMY OF LOCAL CIRCUITS IN BARREL CORTEX
桶状皮层局部电路的功能解剖
- 批准号:
3418746 - 财政年份:1993
- 资助金额:
$ 1.97万 - 项目类别:
FUNCTIONAL ANATOMY OF LOCAL CIRCUITS IN BARREL CORTEX
桶状皮层局部电路的功能解剖
- 批准号:
2269780 - 财政年份:1993
- 资助金额:
$ 1.97万 - 项目类别:
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