ACAT FUNCTIONS USING KNOCKOUT MICE

说明(改编自申请人摘要)酰基辅酶A： 胆固醇酰基转移酶(ACAT)是一种胞内酶 催化胆固醇酯(CE)的形成。这是一种假设 这种酶负责巨噬细胞中CE的合成(在 动脉壁)，用于胆固醇吸收、肝脏极低密度脂蛋白的产生和 肾上腺类固醇激素的生成。为了确定ACAT在这些方面的作用 在这一过程中，PI培育出了ACAT基因敲除小鼠。这些老鼠将被用来 以确定ACATS在这些过程中的作用。将对这些小鼠进行研究 单独或与其他基因敲除小鼠(载脂蛋白E或低密度脂蛋白受体)繁殖后 将被用来确定ACAT是否在 动脉硬化。PI还将表征似乎编码的cDNA 两个新的ACAT新基因。

DESCRIPTION (Adapted from Applicant's Abstract) Acyl coenzyme A: cholesterol acyl transferase (ACAT) is an intracellular enzyme that catalyses the formation of cholesteryl esters (CE). It has been postulated that this enzyme is responsible for the synthesis of CE in macrophages (in the artery wall), for cholesterol absorption, hepatic VLDL production and adrenal steroidogenesis. In order to determine the role of ACAT in these processes, the PI has developed ACAT knockout mice. These mice will be used to determine ACATs role in these processes. These same mice will be studied alone or after breeding with other knockout mice (apoE or LDL Receptor) and will be used to determine whether ACAT has an important role in atherosclerosis. The PI will also characterize cDNAs that appear to encode two new novel ACAT genes.