PHYSIOLOGY OF DROSOPHILA PHOTORECEPTORS

拟议研究的长期目标是获得洞察力 光感受器中的信号转导机制 视觉系统的细胞。 预计结果 从这项研究中获得的信息将有助于了解 神经系统中的感觉接收和信息处理。 该试点研究项目的近期目标是开发 系统中，可以分离果蝇的感光神经元， 在细胞培养物中鉴定并维持， 电生理特性的光激活 水流 将分离的光感受器的细胞培养物 通过酶促和机械解离制备 胚胎、幼虫、蛹和成虫的眼组织。 的身份 培养物中的感光细胞将通过使用 荧光标记感光细胞特异性单克隆抗体 抗体的在旨在确定 感光细胞，Fura-2，荧光Ca++浓度 指示剂，将用于检测光激活的细胞内 其他细胞可能不存在的Ca++瞬变 文化中的类型。 膜片钳记录技术将用于研究 光激活离子通道的生物物理特性 和切除的膜补片。本分析的目的是 识别直接激活细胞内信号的细胞内信使， 无脊椎动物制剂中的光敏通道。

The long range goal of the proposed research is to gain insight into the mechanisms of signal transduction in the photoreceptor cells of the visual system. It is anticipated that the results obtained from this study will aid in understanding the basis of sensory reception and information processing in the nervous system. The immediate goal of this pilot research projects is to a develope system in which isolated photoreceptor neurons of Drosphila can be identified and maintained in cell culture for the purpose of the electrophysiological characterization of the light activated currents. Cell cultures of isolated photoreceptors will be prepared by enzymatic and mechanical dissociation of embryonic,larval, pupal and adult eye tissue. The identity of photoreceptor cells in the cultures will be determined by using fluorescently labeled photoreceptor cell specific monoclonal antibodies. In parallel experiments aimed at identifying the photoreceptor cells, Fura-2, a fluorescent Ca++ concentration indicator, will be used to detect light activated intracellular Ca++ transients which are likely to be absent from the other cell types in the cultures. Patch clamp recording techniques will be used to study the biophysical properties of light activated ion channels in attached and excised membrane patches. The aim of this analysis will be to identify the Intracellular messenger which directly activates the light sensitive channels in the invertebrate preparation.