ELECTROGENERATED CHEMILUMINESCENCE SENSOR ASSEMBLIES

总体目标是开发和演示一种小型光纤 结合电致化学发光（CL）的探针 用于溶液中痕量浓度的测定; 将使用鲁米诺和Ru（bipy）2+ 3CL体系。 接下来要解决的目标是：1）评估定位， 工作电极与光纤束的距离较小 vs.将光学透明电极直接沉积在所述 束的表面。 2)检查溶解CL试剂与 将CL试剂掺入到聚合物薄膜上， 工作电极的表面。 3)审查下列行为的后果： 搅拌溶液而不是让它保持静止。 四、 确定工作范围、检测限和精密度 过氧化氢和鲁米诺的测定（用鲁米诺CL） 草酸根和Ru（bipy）2+3（Ru（bipy）2+3 CL）的测定。 鲁米诺CL已证明适用于测定 可转化为等量氢的物质 过氧化物（如葡萄糖、肌酸酐、胆固醇等）和 在免疫分析中，可以用鲁米诺标记的物质。 Ru（bipy）2+ 3CL可用于测定可氧化物， 草酸盐和胺以及用Ru（bipy）2+3标记的物质 衍生物. CL的电生成允许避免样品 加入催化剂稀释，打开和关闭反应 通过控制电极电位，并物理定位CL 在工作电极表面的发射。 计划是“对样品进行测量。“传感器 直径可以小于5 mm，因此可以在小的 小瓶。 一个传感器计划将使用铂丝电极 位于距离光纤束的端部大约1 mm处; 第二种方法是气相存款一薄层 光学透明的电极材料（氧化锡或金）， 光纤束的抛光表面。 溶液pH值、电极电位、化学发光试剂 浓度，溶解的与聚合物包埋的CL试剂和 将研究静止溶液与搅拌溶液。 测定 鲁米诺（或鲁米诺标记的物质）的双工作电极将 一种用于生成过氧化物试剂，一种用于 电生鲁米诺CL.

The overall aim is to develop and demonstrate a small fiber optic probe that incorporates electrogeneration of chemiluminescence (CL) to be used for determination of trace concentrations in solution; both the luminol and the Ru(bipy)2+3 CL systems will be utilized. Aims to be addressed in turn are: 1) Evaluate positioning the working electrode at a small distance from the fiber optic bundle vs. depositing an optically transparent electrode directly upon the surface of the bundle. 2) Examine use of dissolve CL reagent vs. incorporation of the CL reagent into a thin polymeric film on the surface of the working electrode. 3) Examine consequences of stirring the solution vs. allowing it to remain still. 4) Determine working ranges, detection limits, and precisions for determinations of hydrogen peroxide and luminol (with luminol CL) and determination of oxalate and Ru(bipy)2+3 (with Ru(bipy)2+3 CL). Luminol CL has demonstrated applicability for determination of species that can be converted to an equivalent amount of hydrogen peroxide (e.g. glucose, creatinine, cholesterol, etc.) and for species that can be tagged with luminol as in immunoassay. Ru(bipy)2+3 CL can be used to determine oxidixable species like oxalate and amines and also species tagged with Ru(bipy)2+3 derivatives. Electrogeneration of CL allows one to avoid sample dilution from addition of catalyst, turn the reaction on and off by control of electrode potential, and physically localize CL emission at the working electrode surface. The plan is to "take the measurement to the sample." The sensor could be under 5 mm diameter so could probe a sample in a small vial. One sensor plan would be to use a platinum wire electrode located approximately 1 mm from the end of an optical fiber bundle; a second approach would be to vapor deposit a thin layer of an optically transparent electrode material (tin oxide or gold) onto the polished surface of the fiber optic bundle. The effect of solution pH, electrode potential, CL reagent concentration, dissolved vs. polymer-entrapped CL reagents and still vs. stirred solution will be investigated. For determination of luminol (or luminol-tagged species) dual working electrodes will be used, one to generate the peroxide reagent and one to electrogenerate luminol CL.