Human Tissue Acquisition and Cell Culture Laboratory

核心A：人体组织获取和细胞培养实验室 引言。人体组织的可获得性是每个组织内进行实验的必要条件 研究人员共同拥有多年的实验经验，包括 人体组织样本。该项目20多年来一直保持着良好的工作状态 与教学医院病理科及产科和医院工作人员的关系 妇产科的妇科服务。目前的安排是建立在 历史基础，并根据需要进行修改，以适应项目的特定需求并保持 了解与人体组织的使用和采购有关的合规问题。使用以下组件 管理核心，这是收集、处理和培养人体组织和 CELS已被证明是有儿科和成本效益的。此外，它还提供了收购的一致性和 加工和标准化的组织制备和细胞培养，这对 调查过程。 在这个核心实验室内还保留了一个冷冻组织样本库。存储的 样本包括子宫肌层、子宫内膜、宫颈、胎盘、羊膜、蜕膜和脐带血浆。 在过去几年中收集的；也存储了选定的胎儿组织的样本(12-20周 胎龄)在前一个计划期间根据批准的方案从 选择性堕胎。维护存储的组织、它们的位置和相关收集的数据库 医疗信息。 除了获得供计划项目调查人员使用的组织外，产科 妇科现在能够为研究人员提供部分工资支持，因此这种组织收购 产科和妇科的其他调查人员以及其他 各部门在校园，只要他们有IRB批准的协议。

Core A: Human Tissue Acquisition and Cell Culture Laboratory INTRODUCTION. The availability of human tissues is a necessity for the conduct of experiments within each of the projects, and the investigators collectively have many years of experience with experimentation involving human tissue samples. The Program Project has for more than 20 years maintained an excellent working relationship with the Department of Pathology at our teaching hospital and with the staffs of the obstetric and gynecological services in the Department of Obstetrics and Gynecology. The current arrangement was built on that historical base and modified as needed to accommodate specific needs of the projects and to keep abreast of compliance issues related to use and procurement of human tissues. Working with components of the Administrative Core, this centralized process for collection, processing, and culturing of human tissues and cells has proven to be e'<peditious and cost-effective. In addition, it provides a consistency in acquisition and processing and a standardization of tissue preparation and cell culturing that is extremely beneficial to the investigative process. A repository of frozen tissue samples is also maintained within this Core laboratory. The stored samples include myometrium, endometrium, cervix, placenta, amnion, decidua, and cord blood plasma collected over the last several years; also stored are samples of selected fetal tissues (12-20 weeks gestational age) collected during a previous program under an approved protocol for obtaining tissues from elective abortions. A database is maintained of the stored tissues, their locations, and relevant collection and medical information. In addition to obtaining tissues for use by Program Project investigators, the Department of Obstetrics and Gynecology is now able to fund partial salary support for research personnel so that this tissue acquisition program is available to other investigators in the Department of Obstetrics and Gynecology as well as other departments on campus, provided they have IRB-approved protocols.