Ultraviolet Raman Spectroscopic Studies of Protein and Dynamics

描述（由申请人提供）：蛋白质的动态行为将被研究，使用时间分辨拉曼和红外光谱作为实时结构探针。激光温度跳变技术将应用于蛋白质折叠问题，以表征折叠和展开的最早阶段。t跳研究将扩展到研究与功能相关的蛋白质运动：醇脱氢酶的酶活性、铁蛋白的铁释放和细胞色素c触发的细胞凋亡。将以血红蛋白为范例研究蛋白质变构的动力学。泵探针光谱监测的R-T构象变化将用于定义反应坐标的分子细节，使用定点诱变和金属取代，结合计算建模。通过增加我们对血红蛋白的了解，这些研究可能会改善血液学治疗。

DESCRIPTION (provided by applicant): The dynamical behavior of proteins will be investigated, using time-resolved Raman and infrared spectroscopies as probes of structure in real time. Laser temperature-jump techniques will be applied to the protein folding problem in order to characterize the earliest stages in folding and unfolding. T-jump studies will be extended to investigate protein motions tied to function: enzyme activity in alcohol dehydrogenase, iron release from ferritin, and apoptosis triggering by cytochrome c. The dynamics of protein allostery will be investigated, using hemoglobin as a paradigm. Pump-probe spectroscopic monitoring of the R-T conformation change will be used to define the reaction coordinate in molecular detail, using site-directed mutagenesis and metal substitution, in conjunction with computational modeling. By increasing our understanding of hemoglobin, these studies may lead to improved hematological therapies.