Purchase of a Triple Quadrupole Mass Spectrometer for Multiple Reaction Monitorin

描述(由申请者提供)：本申请申请资助一台三重四极杆质谱仪，该质谱仪具有独特的能力，可以使用一种称为多反应监测(MRM)的方法对特定蛋白质和其他生物分子进行定量。一组非常有经验的研究人员目前可以使用一些高分辨率和串联质谱仪来识别和表征蛋白质结构，他们将使用该仪器和MRM技术进行需要相当高的灵敏度和大量样本的定量测量的目标分析。四个实验室将构成主要用户：罗伯特·J·科特领导的中大西洋质谱学(MAMS)实验室、罗伯特·科尔领导的约翰·霍普金斯高级研究组织蛋白质组学设施，以及阿克希列什·潘迪和杰拉尔德·哈特的研究实验室。重大项目还包括与MAMS和MS/蛋白质组学实验室直接合作的一些研究人员。对于提议使用该仪器的一些项目，MRM提供了一种比这组研究人员目前在遗传学、唐氏综合症、艾滋病毒和痴呆症、糖尿病以及肺癌、胰腺癌和肝癌研究中使用的iTRAQ测量更敏感和选择性更高的方法。此外，该仪器的恒中性损失(CNL)和前体离子扫描模式将用于筛选泛素化和SUMO化蛋白质以及与氧化应激相关的亚硝酸化蛋白质生物标志物。与公共健康相关：该应用程序申请资金用于质谱仪，该质谱仪能够从大量样本中筛选出具有高灵敏度的特定蛋白质和其他生物分子。例如，由疾病和对照个体组成的大型患者队列的结果可用于识别疾病诊断的生物标记物，用于监测治疗结果，或建立设计新药所需的生化途径。这组研究人员将利用该仪器进行遗传学、唐氏综合症、艾滋病毒与痴呆症、糖尿病以及肺癌、胰腺癌和肝癌的研究。

DESCRIPTION (provided by applicant): This application requests funds for a triple quadrupole mass spectrometer with unique capabilities for quantitating specific proteins and other biological molecules using an approach known as multiple reaction monitoring (MRM). A group of very experienced investigators, with access currently to a number of high resolution and tandem mass spectrometers for identifying and characterizing protein structures, will use this instrument and the MRM technique for targeted analyses requiring considerably higher sensitivity and quantitative measurements from large numbers of samples. Four laboratories will constitute the major users: the Middle Atlantic Mass Spectrometry (MAMS) Laboratory directed by Robert J. Cotter, the Johns Hopkins SOM Mass Spectrometry Proteomics Facility directed by Robert Cole, and the research laboratories of Akhilesh Pandey and Gerald Hart. Major projects also include a number of investigators collaborating directly with the MAMS and MS/Proteomics laboratories. For a number of the projects proposed to utilize this instrument, MRM provides a more sensitive and selective approach than the iTRAQ measurements currently used by this group of investigators in studies on genetics, Down syndrome, HIV and dementia, diabetes and cancers of the lung, pancreas and liver. Additionally, the constant neutral loss (CNL) and precursor ion scan modes of this instrument will be used to screen for ubiquitylated and SUMOylated proteins and for nitrosylated protein biomarkers associated with oxidative stress. PUBLIC HEALTH RELEVANCE: This application requests funds for a mass spectrometer capable of screening for specific proteins and other biological molecules with high sensitivity from large numbers of samples. The results from, for example, a large patient cohort composed of disease and control individuals may be used to identify biomarkers for disease diagnosis, for monitoring the outcomes of therapies, or to establish the biochemical pathways needed to design new drugs. This group of investigators will utilize the instrument in studies on genetics, Down syndrome, HIV and dementia, diabetes and cancers of the lung, pancreas and liver.