Olympus FV1000 Scanning Confocal-TIRF Imaging Workstation

描述（由申请人提供）：本提案旨在获得Olympus FV 1000扫描共焦-TIRF成像工作站。该仪器的收购将显着提高蒙大拿大学的研究和教育能力，包括分子组织学和荧光成像（MHFI）和核心设施生物光谱核心研究设施（BCRF）。这两个核心合作，以支持NIH资助和其他研究人员的研究活动，他们参加了UM的三个相互作用的中心：NCRR COBRE结构和功能神经科学中心（CSFN）; NCRR COBRE环境健康科学中心（CEHS）;以及最近成立的生物分子结构和动力学中心（CBSD）。这两个核心设施将其研究，教学和服务活动与其他UM核心研究设施（计算和质谱等）相结合，以提供协同，多学科的方法，并促进大学内外的合作，以解决生物物理学，生物化学和分子生物学的基本问题。UM的仪器能力将对落基山脉西北部及其他地区的生物医学研究产生重大而广泛的影响。公共卫生相关性：拟议的仪器将促进蒙大拿大学三个研究中心的生物医学研究，重点分别是神经生物学，环境健康和生物分子结构和动力学。蒙大拿大学的NIH资助的研究人员将使用这种仪器来成像和分析模型系统和细胞中功能重要的相互作用的动力学。通过这种方式，蛋白质，膜和核酸水平上的相互作用和动力学可以与细胞和组织水平上的事件相关，从而为我们理解疾病和人类生物学调节的核心功能相互作用提供新的见解。

DESCRIPTION (provided by applicant): This proposal is to acquire an Olympus FV1000 Scanning Confocal-TIRF Imaging Workstation. Acquisition of this instrumentation will significantly enhance the research and educational capacity at The University of Montana including the Molecular Histology and Fluorescence Imaging (MHFI) and the Core Facility Biospectroscopy Core Research Facility (BCRF). These two cores collaborate to support the research activities of NIH-funded and other investigators who participate in three interacting centers at UM: the NCRR COBRE Center for Structural and Functional Neuroscience (CSFN); the NCRR COBRE Center for Environmental Health Science (CEHS); and the recently established Center for Biomolecular Structure and Dynamics (CBSD). The two core facilities integrate their research, teaching and service activities with other UM core research facilities (computational, and mass spectrometry, among others) to provide synergistic, multidisciplinary approaches and promote collaborations from within and without the University to address fundamental questions in biophysics, biochemistry and molecular biology. Instrumentation capability at UM will have significant, broad impact on biomedical research in the Rocky Mountain Northwest and beyond. PUBLIC HEALTH RELEVANCE: The proposed instrumentation will facilitate biomedical research in three research centers at The University of Montana with focuses in neurobiology, environmental health, and biomolecular structure and dynamics, respectively. NIH-funded investigators at The University of Montana will use this instrumentation to image and analyze the dynamics of functionally important interactions in model systems and cells. In this way, interactions and dynamics at the protein, membrane, and nucleic acid level can be related to events at the cellular and tissue levels, thus providing new insights about functional interactions central to our understanding of disease and the regulation of human biology.