Multi-color Benchtop Flow Cytometer

描述（由申请人提供）：本申请要求为LSR II流式细胞仪提供资金，用于定量分析在多种流式细胞仪检测中检查的固定和活细胞中的荧光。该仪器将安装在新建的跨学科研究综合体（IRC）中，其实验室空间专为流式细胞术设计，并将成为威斯康星州大学Paul P. Carbone综合癌症中心流式细胞术设施的一部分，该设施为威斯康星州大学多个部门的研究人员提供服务。IRC的使命是让新的科学发现从研究实验室迅速转移到临床护理环境。它将为研究许多与健康相关的细胞机制的医生和科学家提供服务，包括器官排斥，哮喘中的细胞相互作用，癌症发展机制和药物发现。该台式细胞仪将配备高通量筛选装置和405、488、532和640 nm激光器。除了提供新的功能外，细胞仪还将减轻对现有LSRII的需求。该仪器将配备三个目前在UW校园中不可用的组件：一个532 nm激光器，一个407 nm与6个光电倍增管配对，以及一个高通量样品输送设备，该设备将为用户提供快速，自动化的样品采集实验设置在96或348孔板。总之，新的细胞仪将满足威斯康星州校园内NIH资助的广泛科学界的需求，并将扩展当前可用于荧光分析的技术。公共卫生相关性：所要求的多色流式细胞仪将能够同时测量被研究细胞内或细胞上的多达18种不同分子;它将支持许多不同的医生和科学家的工作，他们希望开始了解不同的分子和细胞机制如何在许多不同类型的疾病中共同发挥作用。例如，它将使移植外科医生能够剖析器官排斥的细胞机制，过敏症专家能够了解哮喘的免疫机制，基础科学家能够了解癌症发展的多种机制，并检查许多不同化合物在治疗这些疾病中的作用。将该仪器放置在靠近医院的跨学科研究中心，将使新的科学发现从研究实验室迅速转移到临床护理环境。

DESCRIPTION (provided by applicant): This application requests funding for an LSR II flow cytometer for quantitative analysis of fluorescence in fixed and living cells examined in numerous flow cytometric assays. The instrument will be installed in the newly built Interdisciplinary Research Complex (IRC) with laboratory space designed specifically for flow cytometry and will become part of the University of Wisconsin Paul P. Carbone Comprehensive Cancer Center's flow cytometry facility, which serves investigators from multiple departments throughout the University of Wisconsin research community. The mission of the IRC is to allow new scientific discoveries to move rapidly from research laboratories to clinical care settings. It will serve physicians and scientists that study many health- related cellular mechanisms, including organ rejection, cellular interactions in asthma, mechanisms of cancer development, and drug discovery. This benchtop cytometer will be equipped with a high-throughput screening device and 405, 488, 532, and 640 nm lasers. In addition to providing new features, the cytometer will relieve the demand for the existing LSRII. This instrument will come equipped with three components not currently available on the UW campus: a 532 nm laser, a 407 nm paired with 6 photomultiplier tubes, and a high-throughput sample-delivery device that will provide users with rapid, automated sample acquisition for experiments set-up in 96- or 348-well plates. In summary, the new cytometer will serve the needs of a broad NIH-funded scientific community at the University of Wisconsin campus and will expand the current technology available for fluorescence analysis. Public Health Relevance: The multi-color flow cytometer requested will be able to measure up to 18 different molecules simultaneously in or on the cells being studied; it will support the work of many different physicians and scientists who want to begin to understand how different molecular and cellular mechanisms work together in many different types of diseases. For example, it will enable transplant surgeons to dissect cellular mechanisms of organ rejection, allergists to understand immunological mechanisms in asthma, and basic scientists to understand multiple mechanisms of cancer development and examine the effect of many different compounds in the treatment of these diseases. Placement of this instrument in the Interdisciplinary Research Complex with close proximity to the hospital will allow new scientific discoveries to move rapidly from research laboratories to clinical care settings.