Scanning Laser Confocal for Core Facility

描述（由申请人提供）：校园显微镜和成像设施（CMIF）是俄亥俄州州立大学校园内的核心生物医学显微镜设施。CMIF在2004年获得了一台蔡司510 Meta立式显微镜，并获得了S10的资助，它已经作为主力军工作了四年。蔡司目前每年的使用时间超过2400小时，并且至少提前一周预订。CMIF刚刚搬进了新的十层楼高的生物医学研究塔，该塔将容纳188名研究科学家。虽然蔡司510继续为CMIF服务，但需要第二台常规共焦显微镜来处理增加的使用量。在这个应用程序中，六名NIH资助的研究人员展示了蔡司510如何为他们的研究提供关键数据。仪器要求的扫描激光系统将位于直立支架上，配有高数值油物镜、405 nm二极管激光器、氩激光器、绿色HeNe、红色HeNe、三个PMT（无光谱检测器）和易于使用的软件。蔡司510和FV1000上的演示显示这些仪器符合我们的需求。由于提交申请时（2008年3月）市场的变化，我们正在申请资金购买蔡司或奥林巴斯显微镜，如果申请获得资助，我们将在一年内决定制造商和型号。公共卫生相关性：本申请中要求的显微镜将允许目前资助的NIH研究人员进行关键研究。这项研究涉及的一些健康问题包括：神经肌肉疾病、脊髓损伤、癌症、肺病和骨重吸收疾病。

DESCRIPTION (provided by applicant): Campus Microscopy and Imaging Facility (CMIF) is the core biomedical microscope facility on the campus of the Ohio State University. The CMIF obtained a Zeiss 510 META on an upright microscope with a S10 grant in 2004 and it has served as a workhorse for four years. The Zeiss is currently used more than 2400 hours per year and is reserved for at least a week in advance. The CMIF has just moved into the new ten storeys Biomedical Research Tower which will house 188 research scientists. While the Zeiss 510 continues to serve the CMIF, a second routine confocal microscope is needed to handle the increased usage. In this application six NIH funded investigators show how the Zeiss 510 has given them crucial data for their research. The instrument requested scanning laser system will be on an upright stand, with high numerical oil objectives, 405 nm diode laser, Argon laser, green HeNe, Red HeNe, three PMTs (no spectral detectors) and easy to use software. Demos on the Zeiss 510 and FV1000 showed these instruments to fit our needs. Because of the changes in this market at the time of submission (March 2008), we are requesting funds to purchase either a Zeiss or Olympus microscope and will make the decision as to manufacturer and model in a year, if the application is funded. PUBLIC HEALTH RELEVANCE: The microscope requested in this application will allow currently funded NIH researchers to perform their critical research. Some of the health issues addressed in this research include: neuromuscular disease, spinal cord injury, cancer, lung disease, and bone reabsorption disease.