Molecular Biology and Gene Therapy in Burns and Trauma

描述(由申请者提供)：这一现有培训计划的总体目标是为最多两名接受培训的临床住院医师(主要是外科医生)提供2-3年的重点研究经验，这些住院医师承诺从事创伤、损伤、烧伤或炎症研究。该研究计划将侧重于掌握分子生物学、功能基因组学和基因疗法，因为它应用于炎症研究。该计划将利用医学院在不断扩大的功能基因组学、基因疗法和分子生物学领域的独特优势，以及基础科学家和临床医生之间的合作，致力于培养未来的临床院士。分子生物学和炎症研究之间的接口将针对ARDS、败血症综合征、缺血/再灌注损伤、伤口愈合延迟和烧伤创面，教员将从外科、产科/妇科以及分子遗传学和微生物学系获得资助的基础科学家中挑选，他们将担任受训人员的研究导师。外科、麻醉学、内科和病理学的临床导师 科室将与受训人员和研究人员互动，以确保受训人员接触到炎症研究中具有临床重要性的问题。该计划的总体指导将由首席调查员和执行委员会负责。该奖学金的候选人将在全国范围内和佛罗里达大学医学院招募。执行委员会的成功申请者将确定一名研究和临床导师，他将帮助制定正式的培训计划，并定期审查受训人员的进度。培训计划的大部分将在经验丰富的研究导师的实验室进行，但受训人员也将被要求完成研究伦理和统计方法方面的基本课程工作，并将被鼓励在适当的情况下利用向他们提供的博士课程，获得补充的分子生物学教学培训。此外，受训人员还将参加鲍威尔基因治疗中心和遗传学研究所及其基础科学系的研讨会，以及实验室研究会议。他们还将参加临床研讨会，包括外科和重症监护医学大轮。预计成功的毕业生将拥有足够的研究技能，以竞争炎症研究的过渡资金。 相关性：社会认识到临床医生-科学家在翻译生物学和新技术在临床中的应用中所起的关键作用。我们建议，年轻的有兴趣的临床医生在实验室沉浸2-3年，让具有分子生物学专业知识的成功基础科学家进入实验室，将为从事转化研究的内科医生/科学家的职业生涯奠定坚实的基础。成功的学员将拥有功能基因组学、基因疗法和分子生物学方面的专业知识，以帮助将当前的基因革命转化为创伤实践。

DESCRIPTION (provided by applicant): The overall goal of this existing training program is to provide a focused 2-3 year research experience for up to two clinical residents-in- training (predominantly in surgery) expressing a commitment to a career in trauma, injury, burn or inflammation research. The research program will focus on mastery of molecular biology, functional genomics and gene therapy, as it applies to inflammation research. This program will take advantage of the unique strengths of the College of Medicine in the expanding field of functional genomics, gene therapy and molecular biology, as well as the collaborations between basic scientists and clinicians committed to the training of future clinical academicians. The interface between molecular biology and inflammation research will be targeted to ARDS, sepsis syndromes, ischemia/reperfusion injury, delayed wound healing and the burn wound, The faculty will be drawn from funded basic scientists in the Surgery, Obstetrics/ Gynecology, and Molecular Genetics and Microbiology Departments, who will serve as research mentors to the trainees. Clinical mentors from the Surgery, Anesthesiology, Medicine and Pathology Departments will interact with the trainees and the research faculty to assure that the trainees are being exposed to clinically-important issues in inflammation research. Overall direction of the program will rest with the Principal Investigator and the Executive Committee. Candidates for the fellowship will be recruited nationally and from the University of Florida College of Medicine. Successful applicants with the Executive Committee will identify a research and clinical mentor who will help formulate a formal training program and periodic review of the trainee's progress. The bulk of the training program will be in the laboratory of an experienced research mentor, but trainees will also be required to fulfill basic course work in research ethics and statistical methods, and will be encouraged to obtain supplemental didactic training in molecular biology, taking advantage of Ph.D. programs available to them, if appropriate. Furthermore, trainees are expected to participate in seminars in the Powell Gene Therapy Center and Genetics Institute, and in their basic science departments, as well as laboratory research meetings. They will also be expected to attend clinical seminars, including Surgery and Critical Care Medicine Grand Rounds. It is anticipated that successful graduates will possess sufficient research skills to compete for transitional funding in inflammation research. Relevance: Society recognizes the critical role that clinician-scientists play in translational biology and the application of new technologies to the clinic. We propose that a 2-3 year immersion by a young interested clinician in the laboratory of a successful basic scientist with expertise in molecular biology will provide a sound foundation for a career as a physician/scientist involved in translational research. The successful trainees will have the expertise in functional genomics, gene therapy and molecular biology to help translate the current genetic revolution to trauma practice.