High-Throughput BioMEMS

描述（由申请人提供）：我们将开发用于高通量，高含量，基于细胞的筛选，小型稀有细胞组分的表型和操作，以及流式中1-100个细胞的多重表达分析的方法和并行流动仪器。我们将为蛋白质错误折叠疾病的机制和小分子研究提供组合缩放的方法，特别是神经退行性疾病，如帕金森病（模式生物：酵母）。用于（罕见的）原发性造血干细胞（小鼠）的高含量细胞术研究。

DESCRIPTION (provided by applicant): We will develop methods and parallel flow instrumentation for high-throughput, high- content, cell-based screening, for phenotyping and manipulation of small rare-cell fractions, and for multiplexed expression analysis from 1-100 cells in flow. We will provide means for combinatorial scaling of mechanistic and small-molecule studies of protein misfolding disease, particularly neurodegenerative diseases such as Parkinson's (model organism: yeast). And for high-content cytometry studies with (rare) primary hematopoietic stem cells (mouse). PUBLIC HEALTH RELEVANCE: This program would develop new instrumentation and methods for cell-based high- throughput, high-content screening (HCS). The program addresses bottlenecks that severely limit HCS for (i) scale-up to drug discovery, (ii) for handling of small samples of highly heterogeneous primary cell types; (iii) for finding rare cells (e.g. finding pluripotent cells for cancer diagnostics) and, (iv) image-based sorting/enrichment.