MOLECULAR BIOLOGY MODULE

分子生物学(MB)模块自#年Case VSRC P30赠款的第一个资助期以来一直有效 1997年，在布雷迪-卡尔奈博士担任主任的情况下，以及从1998年到2006年7月，以及卡罗尔·勒基，在人员方面一直保持稳定 作为经理。在上一个供资期间，该部门与专门的动物资源特别密切地合作 模块对超过45个小鼠品系进行基因分型。与本模块以前的功能和 购买ABI Prism 7000 qPCR也已成为该模块的一部分(来自生物信息学模块)，我们 预计该模块的使用率将非常高。。。 MB模块将设在病理研究所的中央VSRC设施中，靠近 学校中的视觉调查员(附录D)。该设备在表格HH中列出，其位置如 相邻的图表。模块经理设计了空间，以最大限度地有效利用主要设备，包括 热循环仪和定量聚合酶链式反应。MB模块负责 提供熟练的技术专长、设备和技术 凝胶的聚合酶链式反应分析/基因分型、定量和成像， 细菌的克隆、菌株的产生及中心储藏 细菌、载体和cdna构建。

The Molecular Biology (MB) Module has been in effect since the first funding period of the Case VSRC P30 grant in 1997, has been stable in personnel, with Dr. Brady-Kalnay as Director, and from 1998 until July, 2006, and Carol Luckey as Manager. In the last funding period, the Module has worked especially closely with the Specialized Animal Resource Module to genotype over 45 mouse strains. Taken together with the previous functions of this Module, and with the acquisition of the ABI Prism 7000 qPCR has also become part of this Module (from the Bioinformatics module), we anticipate that the Module will be very heavily utilized. . . The MB Module will be located in centralized VSRC facilities in the Institute of Pathology, in close proximity to the vision investigators in the School (Appendix D). The equipment is listed in form HH, and is located as shown in the adjacent diagram. The Module Manager designed the space to maximize efficient use of the major equipment, including the thermocycler and qPCR. The MB Module is responsible for providing skilled technical expertise, equipment and techniques for PCR analysis/genotyping, quantification and imaging of gels, cloning, generating bacterial strains and the central storage of bacteria, vectors and cDNA constructs.